Prelog and anti-Prelog stereoselectivity of two ketoreductases from Candida glabrata
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Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in conformity with this interested enantiopreference obtained from experimental tests. The reduction of a series of other substituted aryl ketones was also investigated using the two ketoreductases.
KeywordsAnti-Prelog rule Asymmetric reduction Biocatalysis Ketoreductase Molecular modeling
This work was financially supported by National Scientific Major Program (2010ZX09301-012) and Shenyang Municipal Scientific and Technology Research Fund (F11-243-1-00).
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