Inhibition of Camellia sinensis (L.) O. Kuntze on Microcystis aeruginosa and isolation of the inhibition factors
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Low concentration of tea (Camellia sinensis (L.) O. Kuntze) was shown to inhibit the growth of the toxic cyanobacterium Microcystis aeruginosa. The inhibition efficiency was 40 % at 0.1 g dry tea/L and 90 % at 0.2 g/L after a 12-day culture. All varieties of tea used in the test could inhibit Microcystis growth, in which the inhibitory effect of green tea was greater than that of black tea. Antialgal allelochemicals were isolated from tea by solvent extraction, gel-chromatography and high performance liquid chromatography. Two algal-inhibition compounds were identified by liquid chromatography/mass spectrometry as epigallocatechin-3-gallate, epicatechin-3-gallate respectively. These are the main polyphenols in tea that have inhibitory effects on the growth of cyanobacteria. The combined effect of these polyphenols makes tea a promising source of algicide to inhibit the growth of algal blooms.
KeywordsAlgal bloom control Microcystis aeruginosa Purification Tea polyphenol
This study was funded by the National Postdoctoral Science Foundation (2011M500958), Postdoctoral Science Foundation of Jiangsu Province (1101025B) and Youth Science and Technology Innovation Funds of Nanjing Agricultural University (Y0201100108).
- Choe S, Jung I (2001) Growth inhibition of freshwater algae by ester compounds released from rotted plant. Appl Chem 5(2):204–207Google Scholar
- Inderjit, Dakshini KMM, Einhellig FA (1995) Allelopathy: organisms, processes, and applications. ACS Symposium Series. American Chemical Society, Washington, DCGoogle Scholar
- Rice EL, Lin CY, Huang CY (1980) Effects of decaying rice straw on growth and nitrogen fixation of a blue–green alga. Bot Bull Acad Sinica 21:111–117Google Scholar
- Rippka R, Desrulles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignment, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 11:1–61Google Scholar
- Tan X, Kong FX, Yu Y, Zhang M (2009) Spatio-temporal variations of phytoplankton community composition assayed by morphological observation and photosynthetic pigment analyses in Lake Taihu (China). Afr J Biotechnol 8(19):4977–4982Google Scholar