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Biotechnology Letters

, Volume 35, Issue 7, pp 1079–1083 | Cite as

Biodegradation of 2,4-dichlorophenol by shiitake mushroom (Lentinula edodes) using vanillin as an activator

  • S. Tsujiyama
  • T. Muraoka
  • N. Takada
Original Research Paper

Abstract

The white-rot shiitake mushroom, Lentinula edodes, was used to degrade an environmentally hazardous compound, 2,4-dichlorophenol (DCP), using vanillin as an activator. Vanillin increased the mycelial growth from 74 to 118 mg/150 ml culture and accelerated laccase and Mn-peroxidase production from the maximum on days 24–28 without vanillin to days 10–14. It eliminated 92 % of 100 mM DCP with 50 mg vanillin/l compared with only 15 % without vanillin. GC–MS revealed that a diaryl ether dimer of DCP was formed in the culture without vanillin, whereas dimer formation was diminished with vanillin addition. This indicates that vanillin enhances the degradation of DCP and disrupts the formation of the toxic dimer. Therefore, lignin-derived phenol such as vanillin can be used as natural and eco-friendly activators to control white-rot mushrooms, thereby facilitating the effective degradation of environmentally hazardous compounds.

Keywords

Biodegradation 2,4-Dichlorophenol Lentinula edodes Shiitake Vanillin 

Supplementary material

10529_2013_1179_MOESM1_ESM.doc (2.7 mb)
Supplementary material 1 (DOC 2747 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Graduate School of Life and Environmental ScienceKyoto Prefectural UniversityKyotoJapan
  2. 2.Faculty of AgricultureKyoto Prefectural UniversityKyoto CityJapan
  3. 3.Graduate School of Biological Functional ScienceKyoto Prefectural UniversityKyotoJapan

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