Biotechnology Letters

, Volume 35, Issue 4, pp 647–656

Genome-wide mapping of the ozone-responsive transcriptomes in rice panicle and seed tissues reveals novel insight into their regulatory events

  • Kyoungwon Cho
  • Junko Shibato
  • Akihiro Kubo
  • Yoshihisa Kohno
  • Kouji Satoh
  • Shoshi Kikuchi
  • Ganesh Kumar Agrawal
  • Abhijit Sarkar
  • Randeep Rakwal
Original Research Paper

Abstract

The ‘ozone (O3)-responsive transcriptome’ behavior in the panicles and grains of rice plant was studied individually through high-throughput oligo-DNA microarray technique. O3 differentially and separately regulated 620 and 130 genes in the panicles and grains. Among the O3-responsive genes, 176 and 444 genes were up- and down-regulated in panicle compared to 24 and 106 genes in grain, respectively. Further mapping revealed that the majority of differentially expressed genes were mainly involved in signaling, hormonal, cell wall, transcription, proteolysis, and defense events. Many previously unknown O3-responsive novel genes were identified. Inventory of 745 O3-responsive genes and their mapping will expand our knowledge on novel regulatory processes in both panicles and grains of rice; and, serve as a resource towards the designing of rice crops for future high-O3world.

Purpose of work

Tropospheric ozone (O3) severely affects agricultural production worldwide. Present study aims to reveal a detailed O3 responsive gene network in panicle and grains of rice plants through transcriptomics approach. Our results provide an insight into the basis of O3-response in rice plants, and will help to develop suitable rice genotype for future high O3- world.

Keywords

DNA microarray Grains Oryza sativa L. Ozone Panicle 

Supplementary material

10529_2012_1118_MOESM1_ESM.pptx (2.2 mb)
Supplementary material 1 (PPTX 2,238 kb)
10529_2012_1118_MOESM2_ESM.ppt (152 kb)
Supplementary material 2 (PPT 150 kb)
10529_2012_1118_MOESM3_ESM.pptx (66 kb)
Supplementary material 3 (PPTX 67 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Kyoungwon Cho
    • 1
    • 2
  • Junko Shibato
    • 1
    • 3
  • Akihiro Kubo
    • 1
  • Yoshihisa Kohno
    • 4
  • Kouji Satoh
    • 5
  • Shoshi Kikuchi
    • 5
  • Ganesh Kumar Agrawal
    • 6
  • Abhijit Sarkar
    • 6
    • 7
  • Randeep Rakwal
    • 3
    • 6
    • 8
  1. 1.Environmental Biology DivisionNational Institute for Environmental Studies (NIES)TsukubaJapan
  2. 2.Seoul Center, Korea Basic Science Institute (KBSI)SeoulSouth Korea
  3. 3.Department of AnatomyShowa University School of MedicineShinagawaJapan
  4. 4.Environmental Science Research LaboratoryCentral Research Institute of Electric Power Industry (CRIEPI)ChibaJapan
  5. 5.Plant Genome Research Unit, Division of Genome and Biodiversity ResearchNational Institute of Agrobiological Sciences (NIAS)TsukubaJapan
  6. 6.Research Laboratory for Biotechnology and Biochemistry (RLABB)KathmanduNepal
  7. 7.CSIR—SRF, Laboratory of Air Pollution and Global Climate Change, Department of BotanyBanaras Hindu University (B.H.U.)VaranasiIndia
  8. 8.Graduate School of Life and Environmental Sciences, University of TsukubaTsukubaJapan

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