Overexpression of maize anthocyanin regulatory gene Lc affects rice fertility
- 789 Downloads
Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additionally, these plants exhibited intense pigmentation on the surface of the anther and the bottom of the ovary. They were unable to properly bloom and were completely sterile. Following pollination with normal pollen, these plants yielded red caryopses but did not mature normally. QRT-PCR analysis indicated that mRNA accumulation of the CHS-like gene encoding a chalcone synthase-related protein was increased significantly in the sterile plant. This is the first report to suggest that upregulation of the CHS gene expression may result in rice sterility and affect the normal development of rice seeds.
KeywordsAnthocyanin regulatory gene (Lc) Chalcone sythase-like gene expression Sterility Transgenic rice
We sincerely thank Prof. Zhang Dabing (Shanghai Jiao Tong University) for helping during the course of QRT-PCR detection. This research was supported by the Leading Academic Discipline Project of Shanghai Municipal Education Commission, Project Number: J50401.
- Li JY, Lv YH, Yang LJ et al (2008a) Reducing amylose content of ‘Xiang Qing’ and hybrid rice seeds by introducing anti-waxy gene. Acta Bot Boreal-Occident Sin 28:1082–1087Google Scholar
- Li JY, Xu Y, Wei XL et al (2008b) Changing flower color by introducing the anthocyanin regulatory gene Lc from Maize into tobacco–an experiment for gene engineering teaching. J Shanghai Normal University 37:613–617Google Scholar
- Taylor LP, Jorgensen R (1992) Conditional male fertility in chalcone synthase-deficient petunia. J Heredity 83:11–17Google Scholar
- Wei XL, Liu LN, Wu F et al (2005) The construction of expression vector of flower color regulatory gene (Lc) and transformed into tobacco. J Shanghai Normal University 11(Supplement):135–140Google Scholar