Biotechnology Letters

, Volume 35, Issue 1, pp 115–119 | Cite as

Overexpression of maize anthocyanin regulatory gene Lc affects rice fertility

  • Yuan Li
  • Tao Zhang
  • Zhong-Wei Shen
  • Yu Xu
  • Jian-Yue Li
Original Research Paper


Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additionally, these plants exhibited intense pigmentation on the surface of the anther and the bottom of the ovary. They were unable to properly bloom and were completely sterile. Following pollination with normal pollen, these plants yielded red caryopses but did not mature normally. QRT-PCR analysis indicated that mRNA accumulation of the CHS-like gene encoding a chalcone synthase-related protein was increased significantly in the sterile plant. This is the first report to suggest that upregulation of the CHS gene expression may result in rice sterility and affect the normal development of rice seeds.


Anthocyanin regulatory gene (LcChalcone sythase-like gene expression Sterility Transgenic rice 



We sincerely thank Prof. Zhang Dabing (Shanghai Jiao Tong University) for helping during the course of QRT-PCR detection. This research was supported by the Leading Academic Discipline Project of Shanghai Municipal Education Commission, Project Number: J50401.

Supplementary material

10529_2012_1046_MOESM1_ESM.docx (11.6 mb)
Supplementary material 1 (DOCX 11876 kb)


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yuan Li
    • 1
  • Tao Zhang
    • 1
  • Zhong-Wei Shen
    • 1
  • Yu Xu
    • 1
  • Jian-Yue Li
    • 1
  1. 1.College of Life and Environmental SciencesShanghai Normal UniversityShanghaiPeople’s Republic of China

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