The yeast hypoxic responses, resources for new biotechnological opportunities
- 672 Downloads
Recent advances in the knowledge of molecular mechanisms that control the adaptation to low oxygen levels in yeast and their biotechnological applications, including bioproduct synthesis, such as ethanol, glutathione or recombinant proteins, as well as pathogenic virulence, are reviewed. Possible pathways and target genes, which might be of particular interest for the improvement of biotechnological applications, are evaluated.
KeywordsHeme-sensing Hypoxia Sterol-sensing molecular Yeast bio-productions Yeast pathogen virulence
This research was supported by Grant BFU2009-08854 from Ministerio de Ciencia e Innovación (Spain), co-financed by Fondo Europeo de Desarrollo Regional (FEDER). General support to the laboratory during 2008–11 was funded by Xunta de Galicia (Consolidación Grupos Referencia Competitiva 2008/008), co-financed by FEDER. A.V’s salary was funded by the “María Barbeito pre-doctoral program” from Xunta de Galicia.
- Bao WG, Guiard B, Fang ZA, Donnini C, Gervais M, Passos FM, Ferrero I, Fukuhara H, Bolotin-Fukuhara M (2008) Oxygen-dependent transcriptional regulator Hap1p limits glucose uptake by repressing the expression of the major glucose transporter gene RAG1 in Kluyveromyces lactis. Eukaryot Cell 11:1895–1905CrossRefGoogle Scholar
- Castro Prego R, Lamas Maceiras M, Soengas P, Carneiro I, González Siso MI, Cerdán ME (2010a) Regulatory factors controlling transcription of Saccharomyces cerevisiae IXR1 (ORD1) by oxygen levels. A model of transcriptional adaptation from aerobiosis to hypoxia implicating ROX1 and IXR1 cross-regulation. Biochem J 425:235–243CrossRefGoogle Scholar
- Chantrel Y, Gaisne M, Lions C, Verdière J (1998) The transcriptional regulator Hap1p (Cyp1p) is essential for anaerobic or heme-deficient growth of Saccharomyces cerevisiae: genetic and molecular characterization of an extragenic suppressor that encodes a WD repeat protein. Genetics 148:559–569PubMedGoogle Scholar
- de Groot M, Daran-Lapujade P, van Breukelen B, Knijnenburg T, de Hulster E, Reinders M, Pronk J, Heck A, Slijper M (2007) Quantitative proteomics and transcriptomics of anaerobic and aerobic yeast cultures reveals post-transcriptional regulation of key cellular processes. Microbiology 153:3864–3878PubMedCrossRefGoogle Scholar
- Kandasamy P, Vemula M, Oh CS, Chellappa R, Martin CE (2004) Regulation of unsaturated fatty acid biosynthesis in Saccharomyces: the endoplasmic reticulum membrane protein, Mga2p, a transcription activator of the OLE1 gene, regulates the stability of the OLE1 mRNA through exosome-mediated mechanisms. J Biol Chem 279:36586–36592PubMedCrossRefGoogle Scholar
- Micolonghi C, Ottaviano D, Di Silvio E, Damato G, Heipieper H, Bianchi MM (2012) A dual signaling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of KlMGA2 gene in Kluyveromyces lactis. Microbiology 158:1734–1744PubMedCrossRefGoogle Scholar
- Vukotic M, Oeljeklaus S, Wiese S, Vögtle FN, Meisinger C, Meyer HE, Zieseniss A, Katschinski DM, Jans DC, Jakobs S, Warscheid B, Rehling P, Deckers M (2012) Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab 15:336–347PubMedCrossRefGoogle Scholar
- Walker GM (1998) Yeast metabolism in “Yeast Physiology and Biotechnology”. Wiley, ChichesterGoogle Scholar