Biotechnology Letters

, Volume 34, Issue 11, pp 2087–2091 | Cite as

Type II thioesterase gene (ECO-orf27) from Amycolatopsis orientalis influences production of the polyketide antibiotic, ECO-0501 (LW01)

  • Yang Shen
  • He Huang
  • Li Zhu
  • Minyu Luo
  • Daijie Chen
Original Research Paper
First Online:
Received:
Accepted:

Abstract

ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial for LW01 biosynthesis. ECO-TE I, the gene encoding type I thioesterase from LW01 polyketide synthases, cannot complement ECO-orf27 deficient mutant distinguishing ECO-orf27 from type I thioesterase gene. Type II thioesterase gene pikAV from Streptomyces venezuelae could complement ECO-orf27 in A. orientalis indicating that the two genes are equivalent in their function. Overexpression of ECO-orf27 resulted in a 20 % increase in LW01 production providing an alternative approach for yield improvement.

Keywords

Amycolatopsis orientalis Antibiotic ECO-0501 antibiotic LW01 antibiotic Polyketide synthase Thioesterase 
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Notes

Acknowledgments

The research is granted by the National S&T Major Special Project on Major New Drug Innovation (2009ZX9302-004) and National Natural Science Foundation (NFSC30672558).

Supplementary material

10529_2012_1010_MOESM1_ESM.doc (2.9 mb)
Supplementary material 1 (DOC 2960 kb)

References

  1. Banskota AH, McAlpine JB, Sorensen D, Ibrahim A, Aouidate M, Piraee M, Alarco AM, Farnet CM, Zazopoulos E (2006) Genomic analyses lead to novel secondary metabolites. J Antibiot 59(9):533–542PubMedCrossRefGoogle Scholar
  2. Butler AR, Bate N, Cundliffe E (1999) Impact of thioesterase activity on tylosin biosynthesis in Streptomyces fradiae. Chem Biol 6(5):287–292PubMedCrossRefGoogle Scholar
  3. Claxton HB, Akey DL, Silver MK, Admiraal SJ, Smith JL (2009) Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway. J Biol Chem 284(8):5021–5029PubMedCrossRefGoogle Scholar
  4. Ding X, Tian Y, Chiao J, Zhao G, Jiang W (2003) Stability of plasmid pA387 derivatives in Amylcolatopsis mediterranei producing rifamycin. Biotechnol Lett 25(19):1647–1652PubMedCrossRefGoogle Scholar
  5. Donadio S, Monciardini P, Sosio M (2007) Polyketide synthases and nonribosomal peptide synthetases: the emerging view from bacterial genomics. Nat Prod Rep 24(5):1073–1109PubMedCrossRefGoogle Scholar
  6. Du L, Lou L (2010) PKS and NRPS release mechanisms. Nat Prod Rep 27(2):255–278PubMedCrossRefGoogle Scholar
  7. Gust B, Challis GL, Fowler K, Kieser T, Chater KF (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci 100(4):1541–1546PubMedCrossRefGoogle Scholar
  8. Hertweck C (2009) The biosynthetic logic of polyketide diversity. Angew Chem Int Ed 48(26):4688–4716CrossRefGoogle Scholar
  9. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces genetics. The John Innes Foundation, NorwichGoogle Scholar
  10. Kim BS, Cropp TA, Beck BJ, Sherman DH, Reynolds KA (2002) Biochemical evidence for an editing role of thioesterase II in the biosynthesis of the polyketide pikromycin. J Biol Chem 277(50):48028–48034PubMedCrossRefGoogle Scholar
  11. Lal R, Khanna R, Dhingra N, Khanna M, Lal S (1998) Development of an improved cloning vector and transformation system in Amycolatopsis mediterranei (Nocardia mediterranei). J Antibiot 51(2):161–169PubMedCrossRefGoogle Scholar
  12. McAlpine (2005) Polyene polyketides and methods of production. WO Patent 2005/024025A1, 17 Mar 2005Google Scholar
  13. Staunton J, Weissman KJ (2001) Polyketide biosynthesis: a millennium review. Nat Prod Rep 18(4):380–416PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yang Shen
    • 1
  • He Huang
    • 2
  • Li Zhu
    • 2
    • 3
  • Minyu Luo
    • 3
  • Daijie Chen
    • 1
  1. 1.School of BiotechnologyEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  2. 2.Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiPeople’s Republic of China
  3. 3.School of PharmacyShanghai Jiaotong UniversityShanghaiPeople’s Republic of China

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