Biotechnology Letters

, Volume 34, Issue 9, pp 1679–1686 | Cite as

Production of rare ginsenosides (compound Mc, compound Y and aglycon protopanaxadiol) by β-glucosidase from Dictyoglomus turgidum that hydrolyzes β-linked, but not α-linked, sugars in ginsenosides

Original Research Paper
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Abstract

Optimal hydrolytic activity of β-glucosidase from Dictyoglomus turgidum for the ginsenoside Rd was at pH 5.5 and 80 °C, with a half-life of ~11 h. The enzyme hydrolysed β-linked, but not α-linked, sugar moieties of ginsenosides. It produced the rare ginsenosides, aglycon protopanaxadiol (APPD), compounds Y, and Mc, via three unique transformation pathways: Rb1 → Rd → F2 → compound K → APPD, Rb2 → compound Y, and Rc → compound Mc. The enzyme converted 0.5 mM Rb2 and 0.5 mM Rc to 0.5 mM compound Y and 0.5 mM compound Mc after 3 h, respectively, with molar conversion yields of 100 %.

Keywords

Aglycon protopanaxadiol Compound Mc Compound Y Dictyoglomus turgidum Ginsenoside β-Glucosidase 
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Notes

Acknowledgments

This work was supported by the Basic Research Lab program (No. 2010-0019306), the National Research Foundation, the Ministry of Education, Science and Technology, and by the Next-Generation BioGreen 21 Program grant, Rural Development Administration, Republic of Korea.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Department of Bioscience and BiotechnologyKonkuk UniversityGwangjin-guRepublic of Korea

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