Biotechnology Letters

, Volume 34, Issue 8, pp 1505–1509

Key cytomembrane ABC transporters of Saccharomyces cerevisiae fail to improve the tolerance to d-limonene

Original Research Paper
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Abstract

ATP-binding cassette transporters (ABC) are important detoxification proteins and were proposed to play important roles in monoterpene resistance in Saccharomyces cerevisiae. In this work, the transcriptional levels of typical ABC transporters of S. cerevisiae under 85 mg d-limonene/l were evaluated using real-time quantitative PCR. Only the transcriptional level of PDR5, YOR1 and PDR15 were upregulated but overexpression of these genes in S. cerevisiae failed to improve d-limonene tolerance suggesting that other mechanisms are involved in tolerance of yeast to monoterpenes.

Keywords

ABC transporters Limonene Monoterpene Real-time quantitative PCR Stress 

Supplementary material

10529_2012_931_MOESM1_ESM.doc (80 kb)
Supplementary material 1 (DOC 80 kb)

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina
  2. 2.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  3. 3.National Engineering Laboratory for Cereal Fermentation Technology (NELCF)Jiangnan UniversityWuxiChina

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