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Biotechnology Letters

, Volume 34, Issue 6, pp 1087–1092 | Cite as

Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans

  • Hee Taek Kim
  • Hyeok-Jin Ko
  • Nahyun Kim
  • Duwoon Kim
  • Dongho Lee
  • In-Geol Choi
  • Hee Chul Woo
  • Myoung Dong Kim
  • Kyoung Heon KimEmail author
Original Research Paper

Abstract

A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2–5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na+. The K M , V max , k cat , and k cat /K M values were: 3 mg ml−1, 6.2 U mg−1, 1.9 × 10−2 s−1, and 6.3 × 10−3 mg−1 ml s−1, respectively.

Keywords

Alg7d Endo-type alginate lyase Polysaccharide lyase-7 Saccharophagus degradans 

Notes

Acknowledgment

This work was financially supported by the Ministry for Food, Agriculture, Forestry and Fisheries, Korea. This work was also supported by the Advanced Biomass R&D Center of Korea Grant (2011-0031353) funded by the Ministry of Education, Science and Technology, Korea. Facility support by the Institute of Biomedical Science and Food Safety, Korea University is also acknowledged.

Supplementary material

10529_2012_876_MOESM1_ESM.doc (3.2 mb)
Supplementary material 1 (DOC 3236 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Hee Taek Kim
    • 1
  • Hyeok-Jin Ko
    • 1
  • Nahyun Kim
    • 1
  • Duwoon Kim
    • 2
  • Dongho Lee
    • 1
  • In-Geol Choi
    • 1
  • Hee Chul Woo
    • 2
  • Myoung Dong Kim
    • 3
  • Kyoung Heon Kim
    • 1
    Email author
  1. 1.School of Life Sciences and BiotechnologyKorea UniversitySeoulKorea
  2. 2.Department of Food Science and TechnologyChonnam National UniversityGwangjuKorea
  3. 3.Department of Chemical EngineeringPukyung National UniversityBusanKorea

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