Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans
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A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2–5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na+. The K M , V max , k cat , and k cat /K M values were: 3 mg ml−1, 6.2 U mg−1, 1.9 × 10−2 s−1, and 6.3 × 10−3 mg−1 ml s−1, respectively.
KeywordsAlg7d Endo-type alginate lyase Polysaccharide lyase-7 Saccharophagus degradans
This work was financially supported by the Ministry for Food, Agriculture, Forestry and Fisheries, Korea. This work was also supported by the Advanced Biomass R&D Center of Korea Grant (2011-0031353) funded by the Ministry of Education, Science and Technology, Korea. Facility support by the Institute of Biomedical Science and Food Safety, Korea University is also acknowledged.