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Biotechnology Letters

, Volume 34, Issue 5, pp 805–812 | Cite as

Momilactione B inhibits protein kinase A signaling and reduces tyrosinase-related proteins 1 and 2 expression in melanocytes

  • Ji Hae Lee
  • Boram Cho
  • Hee-jin Jun
  • Woo-Duck Seo
  • Dong-Woo Kim
  • Kang-Jin Cho
  • Sung-Joon LeeEmail author
Original Research Paper

Abstract

Momilactone B (MB) is a terpenoid phytoalexin present in rice bran that exhibits several biological activities. MB reduced the melanin content in B16 melanocytes melanin content and inhibited tyrosinase activities. Using transcriptome analysis, the genes involved in protein kinase A (PKA) signaling were found to be markedly altered. B16 cells stimulated with MB had decreased concentrations of cAMP protein kinase A activity, and cAMP-response element-binding protein which is a key transcription factor for microphthalmia-associated transcription factor (MITF) expression. Accordingly, the expression of MITF and its target genes, which are essential for melanogenesis, were reduced. MB thus exhibits anti-melanogenic effects by repressing tyrosinase enzyme activity and inhibiting the PKA signaling pathway which, in turn, decreases melanogenic gene expression.

Keywords

Momilactone B Melanogenesis Protein kinase A Tyrosinase 

Notes

Acknowledgment

This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ007480)” Rural Development Administration, Republic of Korea.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ji Hae Lee
    • 1
  • Boram Cho
    • 1
  • Hee-jin Jun
    • 1
  • Woo-Duck Seo
    • 2
  • Dong-Woo Kim
    • 3
  • Kang-Jin Cho
    • 4
  • Sung-Joon Lee
    • 1
    Email author
  1. 1.Division of Food Bioscience and Technology, College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Functional CropNational Institute of Crop ScienceMiryangRepublic of Korea
  3. 3.Research CenterNatural F & O Corp.ChuncheonRepublic of Korea
  4. 4.Food Nutrition and Functionality Division, Department of Agrofood ResourcesNational Academy of Agricultural ScienceSuwonRepublic of Korea

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