Wound healing by a 3.2 kDa recombinant polypeptide from velvet antler of Cervus nippon Temminck
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Velvet antler (VA) is used in traditional Chinese medicine to treat a wide range of ailments including the enhancement of wound healing. A 3.2 kDa recombinant polypeptide of VA from sika deer was purified and compared to native polypeptides stimulation growth of NIH3T3 cells. Both stimulated growth in a dose-dependent manner (10–100 μg/ml). To study its wound healing properties, burn-wounded rats were topically administered with recombinant VA polypeptide or native polypeptide. Rats treated with 0.05 and 0.1% (w/w) polypeptides exhibited significant wound healing. As the yield of recombinant polypeptide was 40-fold higher than that of the native polypeptide, it may therefore be a useful biopharmaceutical.
KeywordsNIH3T3 cells Recombinant polypeptide Velvet antler polypeptide Wound healing
We thank Prof. Tiezhong for providing necessary technical help, and special thanks Prof. Colin for providing crucial advice in the process of writing. This work was supported in part by fund from Shenyang Agricultural University, China.
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