Examining the feasibility of bulk commodity production in Escherichia coli
- 890 Downloads
Escherichia coli is currently used by many research institutions and companies around the world as a platform organism for the development of bio-based production processes for bulk biochemicals. A given bulk biochemical bioprocess must be economically competitive with current production routes. Ideally the viability of each bioprocess should be evaluated prior to commencing research, both by metabolic network analysis (to determine the maximum theoretical yield of a given biocatalyst) and by techno-economic analysis (TEA; to determine the conditions required to make the bioprocess cost-competitive). However, these steps are rarely performed. Here we examine theoretical yields and review available TEA for bulk biochemical production in E. coli. In addition, we examine fermentation feedstocks and review recent strain engineering approaches to achieve industrially-relevant production, using examples for which TEA has been performed: ethanol, poly-3-hydroxybutyrate, and 1,3-propanediol.
KeywordsEscherichia coli Industrial biotechnology Metabolic engineering Metabolic network analysis Strain engineering Techno-economic analysis
CEV was supported by a Queensland State Government Smart Futures Fellowship and the Queensland State Government National and International Research Alliance Program. The work of DKM was partly funded by the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U.S. Department of Energy.
- Archer C, Kim J, Jeong H, Park J, Vickers C, Lee S,Nielsen L (2011) The genome sequence of E. coli W ATCC 9637: comparative genome analysis and an improved genome-scale model of E. coli. BMC Genomics 12:9. doi: 10.1186/1471-2164-1112-1189
- Arifin Y, Sabri S, Sugiarto H, Krömer JO, Vickers CE,Nielsen LK (2011) Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxyburyrate (PHB) production from sucrose in fed batch culture. J Biotechnol. doi: 10.1016/j.jbiotec.2011.07.003
- Bruschi M, Boyes S, Sugiarto H, Nielsen LK,Vickers CE (2011) A universal transferrable sucrose utilization approach for non-sucrose-utilizing E. coli strains. Biotech Adv. doi: 10.1016/j.biotechadv.2011.1008.1019
- Edwards MC, Henriksen ED, Yomano LP, Gardner BC, Sharma LN, Ingram LO, Doran Peterson J (2011) Addition of genes for cellobiase and pectinolytic activity in Escherichia coli for fuel ethanol production from pectin-rich lignocellulosic biomass. Appl Environ Microbiol 77:5184–5191PubMedCrossRefGoogle Scholar
- Erickson B, Nelson JE,Winters P (2011) Perspective on opportunities in industrial biotechnology in renewable chemicals. Biotechnol J. doi: 10.1002/biot.201100069
- IEA (2004) Biofuels for transport: an international perspective. International Energy Agency, ParisGoogle Scholar
- Klein-Marcuschamer D, Holmes B, Simmons BA, Blanch HW (2011) Biofuel economics in plant biomass conversion. Wiley, New York, pp 329–354Google Scholar
- Patel M, Crank M, Dornburg V, Hermann B, Roes L, Hüsing B, Overbeek L, Terragni F,Recchia E (2006) Medium and long-term opportunities and risks of the biotechnological production of bulk chemicals from renewable resources—the potential of white biotechnology. European CommissionGoogle Scholar
- Penloglou G, Chatzidoukas C, Kiparissides C (2011) Microbial production of polyhydroxybutyrate with tailor-made properties: an integrated modelling approach and experimental validation. Biotech Adv. doi: 10.1016/j.biotechadv.2011.1006.1021
- Tao L, Aden A (2009) The economics of current and future biofuels. In Vitro Cell Dev Biol 45:199–217Google Scholar
- Yim H, Haselbeck R, Niu W, Pujol-Baxley C, Burgard A, Boldt J, Khandurina J, Trawick JD, Osterhout RE, Stephen R, Estadilla J, Teisan S, Schreyer HB, Andrae S, Yang TH, Lee SY, Burk MJ, Van Dien S (2011) Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol. Nat Chem Biol 7:445–452PubMedCrossRefGoogle Scholar