Enhancing acid tolerance of Leuconostoc mesenteroides with glutathione
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Leuconostoc mesenteroides is a commercially important lactic acid bacterium currently used as a starter for kimchi and kefir. However, its sensitivity to acid stress limits its performance. L. mesenteroides was grown in a medium supplemented with 3.2 or 6.4 mM glutathione (GSH), and cell survival rates were measured during a long-term mild acid challenge (pH 4.0). As a result, GSH was imported by the cells and protected against acid stress; thereafter it was consumed as a nutrient. Acid stress resistance of starter cultures of this bacterium can thus be improved by cultivating it in media supplemented with GSH.
KeywordsAcid stress Cell growth Glutathione Leuconostoc mesenteroides
This study was supported by the Korea Research Foundation Grant (2009-0085901) funded by the Korea Government (MEST).
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