Biotechnology Letters

, Volume 34, Issue 4, pp 683–687 | Cite as

Enhancing acid tolerance of Leuconostoc mesenteroides with glutathione

  • Ji Eun Kim
  • Hyun-Ju Eom
  • Yujin Kim
  • Ji Eun Ahn
  • Jeong Hwan Kim
  • Nam Soo Han
Original Research Paper

Abstract

Leuconostoc mesenteroides is a commercially important lactic acid bacterium currently used as a starter for kimchi and kefir. However, its sensitivity to acid stress limits its performance. L. mesenteroides was grown in a medium supplemented with 3.2 or 6.4 mM glutathione (GSH), and cell survival rates were measured during a long-term mild acid challenge (pH 4.0). As a result, GSH was imported by the cells and protected against acid stress; thereafter it was consumed as a nutrient. Acid stress resistance of starter cultures of this bacterium can thus be improved by cultivating it in media supplemented with GSH.

Keywords

Acid stress Cell growth Glutathione Leuconostoc mesenteroides 

Notes

Acknowledgment

This study was supported by the Korea Research Foundation Grant (2009-0085901) funded by the Korea Government (MEST).

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Ji Eun Kim
    • 1
  • Hyun-Ju Eom
    • 1
  • Yujin Kim
    • 1
  • Ji Eun Ahn
    • 1
  • Jeong Hwan Kim
    • 2
  • Nam Soo Han
    • 1
  1. 1.Department of Food Science and Technology, BK21 Education and Research Center for Advanced Bio-Agriculture TechnologyChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.Department of Food Science and TechnologyGyeongsang National UniversityJinjuRepublic of Korea

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