Biotechnology Letters

, Volume 33, Issue 12, pp 2361–2366 | Cite as

Inducible protein expression in Drosophila Schneider 2 cells using the lac operator–repressor system

  • Motoaki Wakiyama
  • Reiko Muramatsu
  • Yoko Kaitsu
  • Mariko Ikeda
  • Shigeyuki Yokoyama
Original Research Paper


Schneider line 2 cells, derived from Drosophila melanogaster, can be used as a highly versatile gene expression system. Two powerful promoters derived from the actin5C (Ac5) and metallothionein (Mtn) genes are available. The Mtn promoter can be used for the inducible expression of heterologous proteins unsuitable for constitutive expression. However, to circumvent using CuSO4 or CdCl2 as inducers of the Mtn promoter, we created a modified Ac5 promoter, Ac5LacO, in which two short lac operator sequences are embedded. Expression from the Ac5LacO promoter was regulated with co-expression of the lac repressor and IPTG. More than 25-fold induction of firefly luciferase expression was achieved in transient transfection experiments. Furthermore, we demonstrated that the lac operator–repressor regulatory system functioned in chromosomally integrated cell lines.


Drosophila S2 cells lac operator–repressor Inducible expression IPTG 



This work was supported by the Targeted Proteins Research Program (TPRP), the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Motoaki Wakiyama
    • 1
  • Reiko Muramatsu
    • 1
  • Yoko Kaitsu
    • 1
  • Mariko Ikeda
    • 1
  • Shigeyuki Yokoyama
    • 1
    • 2
  1. 1.RIKEN Systems and Structural Biology CenterYokohamaJapan
  2. 2.The University of Tokyo, Graduate School of ScienceTokyoJapan

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