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Biotechnology Letters

, 33:2271 | Cite as

Polysaccharides purified from the submerged culture of Ganoderma formosanum stimulate macrophage activation and protect mice against Listeria monocytogenes infection

  • Cheng-Li Wang
  • Chia-Chen Pi
  • Chu-Wei Kuo
  • Yu-Jing Zhuang
  • Kay-Hooi Khoo
  • Wen-Hsiung Liu
  • Chun-Jen Chen
Original Research Paper

Abstract

The bioactive components of Ganoderma formosanum have not yet been characterized. We investigated the immunomodulatory activities of the extracellular polysaccharides produced from a submerged mycelial culture of G. formosanum. The polysaccharides were mainly composed of d-mannose, d-galactose and d-glucose. After gel filtration chromatography, three polysaccharide fractions (PS-F1, PS-F2 and PS-F3) were purified. PS-F2 stimulated mouse RAW264.7 macrophages to produce TNF-α and nitric oxide, and enhanced the phagocytic activity of macrophages. PS-F2 challenge in mice triggered an acute inflammatory response characterized by the recruitment of neutrophils and monocytes, which protected mice from subsequent infection of Listeria monocytogenes. The results indicate that the heteropolysaccharides produced by G. formosanum can activate the innate immune response on macrophages.

Keywords

Ganoderma formosanum Immunostimulatory Macrophage Polysaccharide 

Notes

Acknowledgments

The authors thank P.Y. Lin, H.M. Lai and T.J. Lu (National Taiwan University) for help with monosaccharide composition analysis. This work was supported by grants from the Council of Agriculture (97AS-3.1.3-FD-Z1 and 98AS-3.1.3-FD-Z1 to C.J.C.) and the National Science Council (NSC96-2313-B-002-060-MY3 and NSC99-2628-B-002-005-MY3 to C.J.C), Taiwan. This work was also supported in part by the National Health Research Institutes (NHRI-EX97-9724SC to C.J.C.), Taiwan.

Supplementary material

10529_2011_697_MOESM1_ESM.doc (188 kb)
Supplementary material 1 (DOC 214 kb)

References

  1. Babineau TJ, Marcello P, Swails W, Kenler A, Bistrian B, Forse RA (1994) Randomized phase I/II trial of a macrophage-specific immunomodulator (PGG-glucan) in high-risk surgical patients. Ann Surg 220:601–609PubMedCrossRefGoogle Scholar
  2. Boh B, Berovic M, Zhang J, Zhi-Bin L (2007) Ganoderma lucidum and its pharmaceutically active compounds. Biotechnol Annu Rev 13:265–301PubMedCrossRefGoogle Scholar
  3. Chan WK, Law HK, Lin ZB, Lau YL, Chan GC (2007) Response of human dendritic cells to different immunomodulatory polysaccharides derived from mushroom and barley. Int Immunol 19:891–899PubMedCrossRefGoogle Scholar
  4. Ding AH, Nathan CF, Stuehr DJ (1988) Release of reactive nitrogen intermediated and reactive oxygen intermediates from mouse peritoneal macrophages: comparison of activating cytokines and evidence for independent production. J Immunol 141:2407–2412PubMedGoogle Scholar
  5. Irinoda K, Masihi KN, Chihara G, Kaneko Y, Katori T (1992) Stimulation of microbicidal host defence mechanisms against aerosol influenza virus infection by lentinan. Int J Immunopharmacol 14:971–977PubMedCrossRefGoogle Scholar
  6. Jeong YT, Yang BK, Jeong SC, Kim SM, Song CH (2008) Ganoderma applanatum: a promising mushroom for antitumor and immunomodulating activity. Phytother Res 22:614–619PubMedCrossRefGoogle Scholar
  7. Latge JP, Kobayashi H, Debeaupuis JP, Diaquin M, Sarfati J, Wieruszeski JM, Parra E, Bouchara JP, Fournet B (1994) Chemical and immunological characterization of the extracellular galactomannan of Aspergillus fumigatus. Infect Immun 62:5424–5433PubMedGoogle Scholar
  8. Lin ZB, Zhang HN (2004) Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms. Acta Pharmacol Sin 25:1387–1395PubMedGoogle Scholar
  9. Lin JM, Lin CC, Chen MF, Ujiie T, Takada A (1995) Radical scavenger and antihepatotoxic activity of Ganoderma formosanum, Ganoderma lucidum and Ganoderma neo-japonicum. J Ethnopharmacol 47:33–41PubMedCrossRefGoogle Scholar
  10. Lin YL, Liang YC, Lee SS, Chiang BL (2005) Polysaccharide purified from Ganoderma lucidum induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and p38 mitogen-activated protein kinase pathways. J Leukoc Biol 78:533–543PubMedCrossRefGoogle Scholar
  11. Marry M, Cavalier DM, Schnurr JK, Netland J, Yang ZY, Pezeshk V, York WS, Pauly M, White AR (2002) Structural characterization of chemically and enzymatically derived standard oligosaccharides isolated from partially purified tamarind xyloglucan. Carbohydr Polym 51:347–356CrossRefGoogle Scholar
  12. Miyazaki T, Nishijima M (1982) Structural examination of an alkali-extracted, water-soluble heteroglycan of the fungus Ganoderma lucidum. Carbohydr Res 109:290–294CrossRefGoogle Scholar
  13. Paterson RRM (2006) Ganoderma-a therapeutic fungal biofactory. Phytochemistry 67:1985–2001PubMedCrossRefGoogle Scholar
  14. Perticarari S, Presani G, Banfi E (1994) A new flow cytometric assay for the evaluation of phagocytosis and the oxidative burst in whole blood. J Immunol Methods 170:117–124PubMedCrossRefGoogle Scholar
  15. Sanodiya BS, Thakur GS, Baghel RK, Prasad GB, Bisen PS (2009) Ganoderma lucidum: a potent pharmacological macrofungus. Curr Pharm Biotechnol 10:717–742PubMedCrossRefGoogle Scholar
  16. Wang YY, Khoo KH, Chen ST, Lin CC, Wong CH, Lin CH (2002) Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities. Bioorgan Med Chem 10:1057–1062CrossRefGoogle Scholar
  17. Young SH, Antonini JM, Roberts JR (2008) Single pre-exposure to a high dose of zymosan enhances lung defense mechanisms and accelerates the pulmonary clearance of a bacterial pathogen in rats. Exp Lung Res 34:559–578PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Cheng-Li Wang
    • 1
  • Chia-Chen Pi
    • 1
  • Chu-Wei Kuo
    • 2
  • Yu-Jing Zhuang
    • 1
  • Kay-Hooi Khoo
    • 2
  • Wen-Hsiung Liu
    • 1
  • Chun-Jen Chen
    • 1
  1. 1.Department of Biochemical Science and TechnologyNational Taiwan UniversityTaipeiTaiwan, ROC
  2. 2.Institute of Biological ChemistryAcademia SinicaTaipeiTaiwan, ROC

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