Polysaccharides purified from the submerged culture of Ganoderma formosanum stimulate macrophage activation and protect mice against Listeria monocytogenes infection
The bioactive components of Ganoderma formosanum have not yet been characterized. We investigated the immunomodulatory activities of the extracellular polysaccharides produced from a submerged mycelial culture of G. formosanum. The polysaccharides were mainly composed of d-mannose, d-galactose and d-glucose. After gel filtration chromatography, three polysaccharide fractions (PS-F1, PS-F2 and PS-F3) were purified. PS-F2 stimulated mouse RAW264.7 macrophages to produce TNF-α and nitric oxide, and enhanced the phagocytic activity of macrophages. PS-F2 challenge in mice triggered an acute inflammatory response characterized by the recruitment of neutrophils and monocytes, which protected mice from subsequent infection of Listeria monocytogenes. The results indicate that the heteropolysaccharides produced by G. formosanum can activate the innate immune response on macrophages.
KeywordsGanoderma formosanum Immunostimulatory Macrophage Polysaccharide
The authors thank P.Y. Lin, H.M. Lai and T.J. Lu (National Taiwan University) for help with monosaccharide composition analysis. This work was supported by grants from the Council of Agriculture (97AS-3.1.3-FD-Z1 and 98AS-3.1.3-FD-Z1 to C.J.C.) and the National Science Council (NSC96-2313-B-002-060-MY3 and NSC99-2628-B-002-005-MY3 to C.J.C), Taiwan. This work was also supported in part by the National Health Research Institutes (NHRI-EX97-9724SC to C.J.C.), Taiwan.
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