Biotechnology Letters

, 33:2027

Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpa armigera larvae

Original Research Paper

DOI: 10.1007/s10529-011-0665-x

Cite this article as:
Upadhyay, S.K. & Singh, P.K. Biotechnol Lett (2011) 33: 2027. doi:10.1007/s10529-011-0665-x

Abstract

Cry1Ac δ-endotoxin produced by Bacillus thuringiensis (Bt) is used as a bio-pesticide for the control of Helicoverpa armigera. Aminopeptidases N (APN) and alkaline phosphatase (ALP) play critical roles in its action against H. armigera larvae. The binding of Cry1Ac with brush border membrane vesicle (BBMV) proteins was increased with the larval development although the sensitivity of larvae to δ-endotoxins decreased. There was higher expression of ALP than APN in early instar larvae with a ~10-fold higher affinity of Cry1Ac towards ALP than to APN. Binding to a specific receptor is therefore more important for the insecticidal activity rather than overall binding to the BBMV proteins. ALP might play a major role in toxicity as compared to APN.

Keywords

Alkaline phosphatase Aminopeptidase Cry1Ac δ-Endotoxin Helicoverpa armigera 

Supplementary material

10529_2011_665_MOESM1_ESM.doc (61 kb)
Supplementary material 1 (DOC 61 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.National Botanical Research Institute, Council of Scientific and Industrial ResearchLucknowIndia

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