Biotechnology Letters

, 33:2073 | Cite as

Overexpression of a novel chrysanthemum NAC transcription factor gene enhances salt tolerance in tobacco

Original Research Paper

Abstract

The plant-specific NAC (for NAM, ATAF1, 2 and CUC2) transcription factors (TFs) have been implicated in different cellular processes involved in stress responses such as cold, high salinity or drought as well as abscisic acid (ABA) signalling. However, the roles of the chrysanthemum NAC TF genes in plant stress responses are still unclear. A full-length cDNA designated DgNAC1, containing a highly conserved N-terminal DNA-binding NAC domain, has been isolated from chrysanthemum by RACE (rapid amplification of cDNA ends). It encodes a protein of 284 amino acids residues (=~32.9 kDa) and theoretical pI of 7.13. The transcript of DgNAC1 was enriched in roots and flowers than in stems and leaves of the adult chrysanthemum plants. The gene expression was strongly induced by ABA, NaCl, drought and cold treatment in the seedlings. Subcellular localization revealed that DgNAC1:GFP fusion protein was preferentially distributed to nucleus. To assess whether DgNAC1 is a practically useful target gene for improving the stress tolerance of chrysanthemum, we ectopically over-expressed the full-length DgNAC1 cDNA in tobacco and found that the 35S:DgNAC1 transgenic tobacco exhibited a markedly increased tolerance to salt. Despite this increased salt stress tolerance, the transgenic tobacco showed no detectable phenotype defects under normal growth conditions. These results proposed that DgNAC1 is appropriate for application in genetic engineering strategies aimed at improving salt stress tolerance in chrysanthemum.

Keywords

Chrysanthemum DgNAC1 Gene expression Salt stress Transgenic tobacco 

Notes

Acknowledgments

This research was supported by the Key Scientific Research Project of Education Department of Sichuan Province (07ZA082, 09ZA065 and 10ZA051). We thank Prof. Tao Wang (State key Laboratories of AgroBiotechnology, China Agricultural University) for providing the pSAT6-GFP-N1 vector.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Qing-Lin Liu
    • 1
  • Ke-Dong Xu
    • 2
  • Liang-Jun Zhao
    • 3
  • Yuan-Zhi Pan
    • 1
  • Bei-Bei Jiang
    • 1
  • Hai-Qing Zhang
    • 1
  • Guang-Li Liu
    • 1
  1. 1.Department of Ornamental HorticultureSichuan Agricultural UniversityChengduPeople’s Republic of China
  2. 2.Key Lab of Plant Genetics & Molecular Breeding of Department of Life ScienceZhoukou Normal UniversityZhoukouPeople’s Republic of China
  3. 3.Department of Ornamental Horticulture and Landscape ArchitectureChina Agricultural UniversityBeijingPeople’s Republic of China

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