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Biotechnology Letters

, Volume 33, Issue 9, pp 1715–1722 | Cite as

H2 inhibits TNF-α-induced lectin-like oxidized LDL receptor-1 expression by inhibiting nuclear factor κB activation in endothelial cells

  • Guohua Song
  • Hua Tian
  • Jia Liu
  • Hongle Zhang
  • Xuejun Sun
  • Shucun Qin
Original Research Paper

Abstract

H2 is a therapeutic antioxidant that can reduce oxidative stress. Oxidized low-density lipoprotein, which plays roles in atherosclerosis, may promote endothelial dysfunction by binding the cell-surface receptor LOX-1. LOX-1 expression can be upregulated by various stimuli, including TNF-α. Thus, we aimed to examine whether the upregulation of LOX-1 by different stimuli could be blocked by H2 in endothelial cells. H2 significantly abolished the upregulation of LOX-1 by different stimuli, including TNF-α, at the protein and mRNA levels. The TNF-α-induced upregulation of LOX-1 was also attenuated by the NF-κB inhibitor N-acetyl-l-cysteine. H2 inhibited the TNF-α-induced activation of NF-κB and the phosphorylation of IκB-α. Furthermore, H2 inhibited the expression of LOX-1 and the activation of NF-κB in apolipoprotein E knockout mice, an animal model of atherosclerosis. Thus, H2 probably inhibits cytokine-induced LOX-1 gene expression by suppressing NF-κB activation.

Keywords

Human umbilical vein endothelial cells H2 Lectin-like oxidized LDL receptor-1 Nuclear factor κB Tumor necrosis factor-α 

Notes

Acknowledgments

This research was supported by the Taishan Scholars Foundation of Shandong Province and by Special Research Funding of Taishan Medical University.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Guohua Song
    • 1
  • Hua Tian
    • 1
  • Jia Liu
    • 1
  • Hongle Zhang
    • 1
  • Xuejun Sun
    • 2
  • Shucun Qin
    • 1
  1. 1.Institute of AtherosclerosisTaiShan Medical UniversityShanDongChina
  2. 2.Department of Diving MedicineSecond Military Medical UniversityShanghaiChina

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