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Biotechnology Letters

, Volume 33, Issue 9, pp 1809–1813 | Cite as

Biosynthesis of 2-amino-2,3-dimethylbutyramide by nitrile hydratase from a newly isolated cyanide-resistant strain of Rhodococcus qingshengii

  • Zhi-Jian Lin
  • Ren-Chao Zheng
  • Yu-Guo Zheng
  • Yin-Chu Shen
Original Research Paper

Abstract

Cells of a new isolate of Rhodococcus qingshengii harboring nitrile hydratase converted 2-amino-2,3-dimethylbutyronitrile into 2-amino-2,3-dimethylbutyramide (ADBA). Cells also hydrated a broad range of substrates including saturated, unsaturated and cyclic aliphatic nitriles. The microorganism tolerated KCN and ADBA up to 5 mM and 40 g/l, respectively. At 10°C, ADBA reached 33.8 g/l (yield 84.5%) which was 2.5 times that at 30°C.

Keywords

2-amino-2,3-dimethylbutyramide Biotransformation Cyanide-resistant capacity Nitrile hydratase Rhodococcus qingshengii 

Notes

Acknowledgments

This work was financially supported by the Major Basic Research Development Program of China (No. 2011CB710800), Natural Sciences Foundation of Zhejiang Province (No. Z4090612) and Doctor Program for High Education of China (No. 20103317120002).

Supplementary material

10529_2011_623_MOESM1_ESM.docx (142 kb)
Supplementary material 1 (DOCX 141 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Zhi-Jian Lin
    • 1
  • Ren-Chao Zheng
    • 1
  • Yu-Guo Zheng
    • 1
  • Yin-Chu Shen
    • 1
  1. 1.Institute of BioengineeringZhijiang University of TechnologyHangzhouPeople’s Republic of China

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