Biosynthesis of 2-amino-2,3-dimethylbutyramide by nitrile hydratase from a newly isolated cyanide-resistant strain of Rhodococcus qingshengii
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Cells of a new isolate of Rhodococcus qingshengii harboring nitrile hydratase converted 2-amino-2,3-dimethylbutyronitrile into 2-amino-2,3-dimethylbutyramide (ADBA). Cells also hydrated a broad range of substrates including saturated, unsaturated and cyclic aliphatic nitriles. The microorganism tolerated KCN and ADBA up to 5 mM and 40 g/l, respectively. At 10°C, ADBA reached 33.8 g/l (yield 84.5%) which was 2.5 times that at 30°C.
Keywords2-amino-2,3-dimethylbutyramide Biotransformation Cyanide-resistant capacity Nitrile hydratase Rhodococcus qingshengii
This work was financially supported by the Major Basic Research Development Program of China (No. 2011CB710800), Natural Sciences Foundation of Zhejiang Province (No. Z4090612) and Doctor Program for High Education of China (No. 20103317120002).
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