In vivo O2 measurement inside single photosynthetic cells
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The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.
KeywordsSingle cell analysis Oxygen measurement Ultra-micro electrode Amperometry Electrochemical impedance spectroscopy
This work was supported by the Global Climate and Energy Project at Stanford University. S. Bai acknowledges additional support by Ministry of Environment under Environment Convergence Technology Project.