Biotechnology Letters

, Volume 33, Issue 8, pp 1675–1681 | Cite as

In vivo O2 measurement inside single photosynthetic cells

  • Seoung-Jai Bai
  • WonHyoung Ryu
  • Rainer J. Fasching
  • Arthur R. Grossman
  • Fritz B. Prinz
Original Research Paper

Abstract

The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.

Keywords

Single cell analysis Oxygen measurement Ultra-micro electrode Amperometry Electrochemical impedance spectroscopy 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Seoung-Jai Bai
    • 1
  • WonHyoung Ryu
    • 2
  • Rainer J. Fasching
    • 3
  • Arthur R. Grossman
    • 5
  • Fritz B. Prinz
    • 4
  1. 1.Department of Mechanical EngineeringDankook UniversityYong-inRepublic of Korea
  2. 2.School of Mechanical EngineeringYonsei UniversitySeoulRepublic of Korea
  3. 3.Nanoscale Prototyping Laboratory for Energy Conversion and Storage, Department of Mechanical EngineeringStanford UniversityCaliforniaUSA
  4. 4.Department of Material Science and EngineeringStanford UniversityCaliforniaUSA
  5. 5.Department of Plant Biology, Carnegie Institution and Department of Biological SciencesStanford UniversityCaliforniaUSA

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