Biotechnology Letters

, Volume 33, Issue 7, pp 1465–1471 | Cite as

PzsS3a, a novel endosperm specific promoter from maize (Zea mays L.) induced by ABA

  • Yu-Feng Hu
  • Yang-ping Li
  • Junjie Zhang
  • Hanmei Liu
  • Zhiyu Chen
  • Yubi Huang
Original Research Paper

Abstract

The maize zsS3a gene codes for starch synthase. Transcriptional analysis revealed that it is mainly expressed in endosperm and is induced by abscisic acid (ABA). The 5′-flanking region of zsS3a was isolated, and a 1772 bp zsS3a promoter (PzsS3a) was fused to a Luc reporter gene with a maize Adh1 intron. Transient expression assay by bombardment transformation showed that, although the addition of the Adh1 intron enhanced the promoter activity approx. 52-fold, it did not alter the promoter specificity. PzsS3a with the Adh1 intron drove the Luc gene preferentially and it was highly expressed in the endosperm relative to the embryo but not in the leaf or root. Furthermore, the promoter activity in the endosperm was enhanced four fold by 100 mM ABA.

Keywords

Abscisic acid Endosperm Maize Promoter zsS3a Gene 

Notes

Acknowledgments

This work was financially supported by grants from High-tech Research Plan of China (863) (2008AA863019) and the National Major Special Project of China on New Varieties Cultivation for Transgenic Organisms (2009ZX08003-022B).

Supplementary material

10529_2011_582_MOESM1_ESM.docx (191 kb)
Supplementary material 1 (DOCX 191 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Yu-Feng Hu
    • 1
  • Yang-ping Li
    • 1
  • Junjie Zhang
    • 2
  • Hanmei Liu
    • 2
  • Zhiyu Chen
    • 2
  • Yubi Huang
    • 1
    • 3
  1. 1.Maize Research InstituteSichuan Agricultural UniversityYa’anChina
  2. 2.College of Life ScienceSichuan Agricultural UniversityYa’anChina
  3. 3.College of AgronomySichuan Agricultural UniversityYa’anChina

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