A labile point in mutant amphotericin polyketide synthases
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Streptomyces nodosus produces the antifungal polyene amphotericin B. Numerous modifications of the amphotericin polyketide synthase have yielded new analogues. However, previous inactivation of the ketoreductase in module 10 resulted in biosynthesis of truncated polyketides. Here we show that modules downstream of this domain remain intact. Therefore, loss of ketoreductase-10 activity is sufficient to cause early chain termination. This modification creates a labile point in cycle 11 of the polyketide biosynthetic pathway. Non-extendable intermediates are released to accumulate as polyenyl-pyrones.
KeywordsAmphotericin B Polyketide synthase Ketoreductase Polyenyl-pyrone
NK received a PhD studentship from the Irish Higher Education Authority Programme for Research in Third Level Institutions. PC received the financial support of Science Foundation Ireland under Grant number 09/RFP/GEN2132.
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