Biotechnology Letters

, Volume 33, Issue 5, pp 1007–1011

Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification

  • Annette Lin Luhe
  • Lily Tan
  • Jinchuan Wu
  • Hua Zhao
Original Research Paper

DOI: 10.1007/s10529-011-0518-7

Cite this article as:
Luhe, A.L., Tan, L., Wu, J. et al. Biotechnol Lett (2011) 33: 1007. doi:10.1007/s10529-011-0518-7

Abstract

Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l−1 compared to untransformed yeast which grew only at 6% (v/v) ethanol and 100 g glucose l−1.

Keywords

Ethanol tolerance Saccharomyces cerevisiae Whole genome amplification 

Supplementary material

10529_2011_518_MOESM1_ESM.doc (83 kb)
(DOC 83 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Annette Lin Luhe
    • 1
  • Lily Tan
    • 1
  • Jinchuan Wu
    • 1
  • Hua Zhao
    • 1
  1. 1.Industrial Biotechnology Program, Institute of Chemical and Engineering SciencesAgency for Science, Technology and Research (A*STAR)Jurong IslandSingapore

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