Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification
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- Luhe, A.L., Tan, L., Wu, J. et al. Biotechnol Lett (2011) 33: 1007. doi:10.1007/s10529-011-0518-7
Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l−1 compared to untransformed yeast which grew only at 6% (v/v) ethanol and 100 g glucose l−1.