Biotechnology Letters

, Volume 33, Issue 5, pp 883–888 | Cite as

Efficient electroporation of peptides into adherent cells: investigation of the role of mechano-growth factor in chondrocyte culture

  • Claudio Schönenberger
  • Anette Schütz
  • Alfredo Franco-Obregón
  • Marcy Zenobi-Wong
Original Research Paper

Abstract

Peptide therapeutics are of increasing interest due to their biological specificity. We used a simple technique to study the efficacy of inducing peptides into adherent chondrocytes by transiently permeabilizing the membrane with electric pulses (in situ electroporation). Mechano-growth factor (MGF) was selected as a model peptide. FITC-labeled MGF was added to cultures of adherent primary chondrocytes grown on ITO coated glass slides. Cells were subjected to 3–9 pulses of 175–275 V and evaluated by flow cytometry. Under optimal conditions, an electroporation efficiency of close to 50% could be achieved. This technique can be used to study the functional domains of intracellular peptides, peptide inhibition of signal transduction and intracrine-mediated effects of peptides in adherent cells.

Keywords

Chondrocytes In situ electroporation Indium tin oxide Mechano-growth factor Peptide therapeutics 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Claudio Schönenberger
    • 1
  • Anette Schütz
    • 1
  • Alfredo Franco-Obregón
    • 1
  • Marcy Zenobi-Wong
    • 1
  1. 1.Laboratory for Biosensors and BioelectronicsInstitute for Biomedical Engineering, ETZ F84, ETH ZürichZurichSwitzerland

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