Biotechnology Letters

, Volume 33, Issue 2, pp 277–284 | Cite as

Improvement of acetic acid tolerance and fermentation performance of Saccharomyces cerevisiae by disruption of the FPS1 aquaglyceroporin gene

  • Jun-Guo Zhang
  • Xiu-Ying Liu
  • Xiu-Ping HeEmail author
  • Xue-Na Guo
  • Ying Lu
  • Bo-run ZhangEmail author
Original Research Paper


The FPS1 gene coding for the Fps1p aquaglyceroporin protein of an industrial strain of Saccharomyces cerevisiae was disrupted by inserting CUP1 gene. Wild-type strain, CE25, could only grow on YPD medium containing less than 0.45% (v/v) acetic acid, while recombinant strain T12 with FPS1 disruption could grow on YPD medium with 0.6% (v/v) acetic acid. Under 0.4% (v/v) acetic acid stress (pH 4.26), ethanol production and cell growth rates of T12 were 1.7 ± 0.1 and 0.061 ± 0.003 g/l h, while those of CE25 were 1.2 ± 0.1 and 0.048 ± 0.003 g/l h, respectively. FPS1 gene disruption in an industrial ethanologenic yeast thus increases cell growth and ethanol yield under acetic acid stress, which suggests the potential utility of FPS1 gene disruption for bioethanol production from renewable resources such as lignocelluloses.


Acetic acid tolerance Alcoholic fermentation FPS1 disruption Industrial diploid ethanologenic yeast 



The authors would like to acknowledge the financial support of Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX1-YW-11-C4).


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.The Laboratory of Molecular Genetics and Breeding of YeastInstitute of Microbiology, Chinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Graduate University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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