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Biotechnology Letters

, Volume 33, Issue 2, pp 215–220 | Cite as

Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection

  • Xiaofeng RenEmail author
  • Siqingaowa Suo
  • Yong-Suk Jang
Original Research Paper

Abstract

A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-M antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.

Keywords

Coronavirus M protein Porcine epidemic diarrhea virus 

Notes

Acknowledgments

Funding supported by Program for New Century Excellent Talents in Heilongjiang Provincial University (1155–NCET–005), Heilongjiang Provincial Science and Technology Department (ZJN0702-01) and National Natural Science Foundations of China (30700590; 30700591; 30972195) are acknowledged.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Preventive Veterinary MedicineCollege of Veterinary Medicine, Northeast Agricultural UniversityHarbinChina
  2. 2.Division of Biological SciencesChonbuk National UniversityJeonjuRepublic of Korea

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