Biotechnology Letters

, Volume 32, Issue 12, pp 1915–1920 | Cite as

Characterization of a thermostable xylanase from an alkaliphilic Bacillus sp.

  • Guimin Zhang
  • Liangwei Mao
  • Yueju Zhao
  • Yanfen Xue
  • Yanhe Ma
Original Research Paper

Abstract

A xylanase gene (xyn10) from alkaliphilic Bacillus sp. N16-5 was cloned and expressed in Pichia pastoris. The deduced amino acid sequence has 85% identity with xylanase xyn10A from B. halodurans and contains two potential N-glycosylation sites. The glycosylated Xyn10 with MW 48 kDa can hydrolyze birchwood and oatspelt xylan. The enzyme had optimum activity at pH 7 and 70°C, with the specific activity of 92.5U/mg. The Xyn10 retained over 90% residual activity at 60°C for 30 min but lost all activity at 80°C over 15 min. Most tested ions showed no or slight inhibition effects on enzyme activity.

Keywords

Bacillus sp. Heterologous expression Pichia pastoris Thermostable xylanase 

Supplementary material

10529_2010_372_MOESM1_ESM.doc (70 kb)
Supplementary material 1 (DOC 70 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Guimin Zhang
    • 1
    • 2
  • Liangwei Mao
    • 2
  • Yueju Zhao
    • 1
  • Yanfen Xue
    • 1
  • Yanhe Ma
    • 1
  1. 1.State Key Laboratory of Microbial Resources, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  2. 2.College of Life ScienceHubei UniversityWuhanChina

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