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Biotechnology Letters

, Volume 32, Issue 12, pp 1915–1920 | Cite as

Characterization of a thermostable xylanase from an alkaliphilic Bacillus sp.

  • Guimin Zhang
  • Liangwei Mao
  • Yueju Zhao
  • Yanfen Xue
  • Yanhe MaEmail author
Original Research Paper

Abstract

A xylanase gene (xyn10) from alkaliphilic Bacillus sp. N16-5 was cloned and expressed in Pichia pastoris. The deduced amino acid sequence has 85% identity with xylanase xyn10A from B. halodurans and contains two potential N-glycosylation sites. The glycosylated Xyn10 with MW 48 kDa can hydrolyze birchwood and oatspelt xylan. The enzyme had optimum activity at pH 7 and 70°C, with the specific activity of 92.5U/mg. The Xyn10 retained over 90% residual activity at 60°C for 30 min but lost all activity at 80°C over 15 min. Most tested ions showed no or slight inhibition effects on enzyme activity.

Keywords

Bacillus sp. Heterologous expression Pichia pastoris Thermostable xylanase 

Notes

Acknowledgments

This study was supported by the Ministry of Sciences and Technology of China (973 programs 2007CB707801, 863 programs 2006AA020201 and 2007AA021306) and Hubei Province Nature Science Foundation (2008CDB058).

Supplementary material

10529_2010_372_MOESM1_ESM.doc (70 kb)
Supplementary material 1 (DOC 70 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Guimin Zhang
    • 1
    • 2
  • Liangwei Mao
    • 2
  • Yueju Zhao
    • 1
  • Yanfen Xue
    • 1
  • Yanhe Ma
    • 1
    Email author
  1. 1.State Key Laboratory of Microbial Resources, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  2. 2.College of Life ScienceHubei UniversityWuhanChina

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