Cyanobactericidal effect of Rhodococcus sp. isolated from eutrophic lake on Microcystis sp
- 370 Downloads
A bacterium, which was observed in all cultivations of Microcystis sp., was isolated and designated as Rhodococcus sp. KWR2. The growth of bloom-forming cyanobacteria, including four strains of Microcystis aeruginosa and Anabaena variabilis, was suppressed by up to 75–88% by 2% (v/v) culture broth of KWR2 after 5 days. But KWR2 did not inhibit eukaryotic algae, Chlorella vulgaris and Scenedesmus sp. An extracellular algicidal substance produced by KWR2 showed a cyanobactericidal activity of 94% and was water-soluble with a molecular weight of lower than 8 kDa.
KeywordsAlgicide Bloom Cyanobacteria Microcystis Rhodococcus
This work was supported by grants from the Korea Research Institute of Bioscience and Biotechnology Research Initiative Program, the MEST/NRF to the Environment Biotechnology National Core Research Center (grant #: 20090091489), and to the Center for Aquatic Ecosystem Restoration, Eco-STAR project from the Ministry of Environment, Republic of Korea.
- Doi RH (1983) Chromosomal DNA techniques. In: Rodriguez RL (ed) Recombinant DNA techniques. Addison-Wesley, MA, pp 161–163Google Scholar
- Doucette GJ, Kodama M, Franca S, Gallacher S (1998) Bacterial interactions with harmful algal bloom species: bloom ecology toxigenesis and cytology. In: Anderson DM (ed) Physiological ecology of harmful algal blooms. Springer-Verlag, Berlin, pp 619–647Google Scholar
- Fukami K, Yuzawa A, Nishijima T, Hata Y (1992) Isolation and properties of a bacterium inhibiting the growth of Gymnodinium nagasakiense. Nippon Suisan Gakkaishi 58:1073–1077Google Scholar
- Gotham IJ, Rhee GY (1982) Effects of nitrate and phosphate limitation on cyclostat growth of two freshwater diatoms. J Gen Microbiol 128:199–205Google Scholar
- McGuire MJ, Jones RM, Means EG, Izaguirre G, Preston AE (1984) Controlling attached blue-green algae with copper sulfate. J Am Water Works Assoc 76:60–65Google Scholar
- Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Genetic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61Google Scholar