Biotechnology Letters

, Volume 32, Issue 11, pp 1685–1691 | Cite as

Functional expression and magnetic nanoparticle-based Immobilization of a protein-engineered marine fish epoxide hydrolase of Mugil cephalus for enantioselective hydrolysis of racemic styrene oxide

  • Sung Hee Choi
  • Hee Sook Kim
  • In Su Lee
  • Eun Yeol Lee
Original Research Paper


A triple-point mutated fish microsomal epoxide hydrolase (mEH) gene from Mugil cephalus was expressed in Escherichia coli in the presence of various chaperones to prevent protein aggregations. The enantioselective hydrolytic activity was more than doubled by co-expressing the EH mutant gene with pGro7 plasmid. The highly active EH mutant with a his-tag was immobilized onto magnetic silica assembled with NiO nanoparticles. The immobilized mEH mutant was re-used more than 10 times with less than 10% activity loss. (S)-Styrene oxide with 98% enantiopurity was repeatedly obtained with over 50% of the theoretical yield by the magnetically separable high-performance mEH mutant.


Chaperone Chiral epoxides Epoxide hydrolase Immobilization Mugil cephalus 



This work was supported by the Marine and Extreme Genome Research Center Program, Ministry of Land, Transportation and Martime Affairs, Republic of Korea.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Sung Hee Choi
    • 1
  • Hee Sook Kim
    • 1
  • In Su Lee
    • 2
  • Eun Yeol Lee
    • 3
  1. 1.Department of Food Science and BiotechnologyKyungsung UniversityBusanSouth Korea
  2. 2.Department of Applied ChemistryKyung Hee UniversityGyeonggi-doKorea
  3. 3.Department of Chemical Engineering and Industrial Liaison Research InstituteKyung Hee UniversityGyeonggi-doKorea

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