Biotechnology Letters

, Volume 32, Issue 10, pp 1509–1514 | Cite as

Sophorolipid production by Candida bombicola on oils with a special fatty acid composition and their consequences on cell viability

  • I. N. A. Van Bogaert
  • S. Roelants
  • D. Develter
  • W. Soetaert
Original Research Paper

Abstract

Sophorolipids production by the yeast Candia bombicola is most favourable when glucose is used as a carbon source in combination with a hydrophobic carbon source such as a common vegetable oil. Most vegetable oils are comprised of C16–C18 fatty acids, an ideal range for sophorolipid production. The use of oils with either shorter or longer fatty acids, such has coconut oil or meadowfoam oil, respectively, was evaluated. Such oils did not contribute to enhanced sophorolipid production when compared to cultures run on glucose as the sole carbon source. Moreover, a toxic effect of medium-chain fatty acids towards stationary C. bombicola cells was demonstrated.

Keywords

Candida bombicola Coconut oil Meadowfoam oil Medium-chain fatty acids Toxicity of free fatty acids Sophorolipids 

Notes

Acknowledgments

The authors wish to thank Ecover Belgium NV and the Bijzonder Onderzoekfonds of Ghent University for financial support (grants A05/003 and 01D18604).

References

  1. Bergsson G, Arnfinnsson J, Steingrimsson O, Thormar H (2001) In vitro killing of Candida albicans by fatty acids and monoglycerides. Antimicrob Agents Chemother 45:3209–3212CrossRefPubMedGoogle Scholar
  2. Casas JA, Garcia-Ochoa F (1999) Sophorolipid production by Candida bombicola: medium composition and culture methods. J Biosci Bioeng 88:488–494CrossRefPubMedGoogle Scholar
  3. Chen J, Song X, Zhang H, Qu YB, Miao JY (2006) Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae. Enzyme Microb Technol 39:501–506CrossRefGoogle Scholar
  4. Davila AM, Marchal R, Vandecasteele JP (1994) Sophorose lipid production from lipidic precursors—predictive evaluation of industrial substrates. J Ind Microbiol 13:249–257CrossRefGoogle Scholar
  5. Fleuracker SJJ, Van Bogaert INA, Develter D (2010) On the production and identification of medium-chained sophorolipids. Eur J Lipid Sci Technol. doi:10.1002/ejlt.200900158
  6. Gorin PAJ, Spencer JFT, Tulloch AP (1961) Hydroxy fatty acid glycosides of sophorose from Torulopsis magnoliae. Can J Chem 39:846–855CrossRefGoogle Scholar
  7. Konishi M, Fukuoka T, Morita T, Imura T, Kitamoto D (2008) Production of new types of sophorolipids by Candida batistae. J Oleo Sci 57:359–369PubMedGoogle Scholar
  8. Lang S, Brakemeier A, Heckmann R, Spockner S, Rau U (2000) Production of native and modified sophorose lipids. Chim Oggi-Chem Today 18:76–79Google Scholar
  9. Ogawa S, Ota Y (2000) Influence of exogenous natural oils on the omega-1 and omega-2 hydroxy fatty acid moiety of sophorose lipid produced by Candida bombicola. Biosci Biotechnol Biochem 64:2466–2468CrossRefPubMedGoogle Scholar
  10. Spencer JFT, Gorin PAJ, Tulloch AP (1970) Torulopsis bombicola sp. n. Antonie Van Leeuwenhoek 36:129–133CrossRefPubMedGoogle Scholar
  11. Tulloch AP, Spencer JFT, Deinema MH (1968) A new hydroxy fatty acid sophoroside from Candida bogoriensis. Can J Chem 46:345–348CrossRefGoogle Scholar
  12. Van Bogaert INA, Saerens K, De Muynck C, Develter D, Soetaert W, Vandamme EJ (2007) Microbial production and application of sophorolipids. Appl Microbiol Biotechnol 76:23–34CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • I. N. A. Van Bogaert
    • 1
  • S. Roelants
    • 1
  • D. Develter
    • 2
  • W. Soetaert
    • 1
  1. 1.Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience EngineeringGhent UniversityGhentBelgium
  2. 2.Ecover Belgium NVMalleBelgium

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