Biotechnology Letters

, Volume 32, Issue 9, pp 1339–1346

Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems

  • Liangming Zhang
  • Peiqiang Su
  • Caixia Xu
  • Junlin Yang
  • Weihua Yu
  • Dongsheng Huang
Original Research Paper

DOI: 10.1007/s10529-010-0293-x

Cite this article as:
Zhang, L., Su, P., Xu, C. et al. Biotechnol Lett (2010) 32: 1339. doi:10.1007/s10529-010-0293-x

Abstract

High-density cell culture is pivotal for the chondrogenic differentiation of human mesenchymal stem cells (hMSCs). Two high-density cell culture systems, micromass and pellet culture, have been used to induce chondrogenic differentiation of hMSCs. In micromass culture, the induced-cartilage tissues were larger, more homogenous and enriched in cartilage-specific collagen II but the fibrocartilage-like feature, collagen I, and hypertrophic chondrocyte feature, collagen X, were markedly decreased compared to those in pellet culture. Furthermore, real time RT-PCR analysis demonstrated that collagen II and aggrecan mRNA were up-regulated while collagen X and collagen I mRNA were down-regulated in micromass culture. Thus, the micromass culture system is a promising tool for in vitro chondrogenic studies.

Keywords

Chondrogenesis Mesenchymal stem cells Micromass culture Pellet culture Tissue engineering 

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Liangming Zhang
    • 1
  • Peiqiang Su
    • 2
  • Caixia Xu
    • 3
  • Junlin Yang
    • 2
  • Weihua Yu
    • 4
  • Dongsheng Huang
    • 1
  1. 1.Department of Orthopedics, Second Affiliated HospitalSun Yat-Sen UniversityGuangzhouChina
  2. 2.Department of Orthopedics, First Affiliated HospitalSun Yat-Sen UniversityGuangzhouChina
  3. 3.School of Materials Science and EngineeringSouth China University of TechnologyGuangzhouChina
  4. 4.Center for Stem Cell Biology and Tissue EngineeringSun Yat-Sen UniversityGuangzhouChina

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