Biotechnology Letters

, Volume 32, Issue 9, pp 1271–1277 | Cite as

Improvement of human interferon alpha secretion by Lactococcus lactis

  • Qiuxiang Zhang
  • Jin ZhongEmail author
  • Xiaobo Liang
  • Wenjun Liu
  • Liandong HuanEmail author
Original Research Paper


To improve the secretion and expression of human interferon alpha 2b (IFN) in Lactococcus lactis, a synthetic pro-peptide, LEISSTCDA (LEISS), was fused to the N-terminus of IFN. This gave a higher secretion efficiency (12% vs. 5%) and yield (~2.8-fold) of IFN. The signal peptide, SPSlpA (SlpA, an S-layer protein of Lactobacillus brevis), was also tested to secrete IFN instead of SPUsp45 (Usp45, the main secreted protein in L. lactis). This gave increased IFN secretion (~3-fold) but lower total production. All the recombinant IFN had appropriate bioactivities in an antiviral assay.


Human interferon alpha Lactococcus lactis LEISSTCDA Secretion 



This work was supported by grants from National Program for High Technology Research and Development of China (2006AA10Z319) and the Key Project of the Chinese Academy of Sciences (KSCXZ-YW-G-016). We greatly appreciate Ms. Ling Fu and Dr. Xiaoling Liu for their kindly help for analysis of the biological activity of IFN.

Supplementary material

10529_2010_285_MOESM1_ESM.doc (39 kb)
Supplementary material 1 (DOC 39 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Resources, Institute of MicrobiologyChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Graduate University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Center for Molecular Virology, Institute of Microbiology, Chinese Academy of SciencesBeijingPeople’s Republic of China
  4. 4.Department of Pediatric DentistryUniversity of Alabama at BirminghamBirminghamUSA

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