Improvement of human interferon alpha secretion by Lactococcus lactis
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To improve the secretion and expression of human interferon alpha 2b (IFN) in Lactococcus lactis, a synthetic pro-peptide, LEISSTCDA (LEISS), was fused to the N-terminus of IFN. This gave a higher secretion efficiency (12% vs. 5%) and yield (~2.8-fold) of IFN. The signal peptide, SPSlpA (SlpA, an S-layer protein of Lactobacillus brevis), was also tested to secrete IFN instead of SPUsp45 (Usp45, the main secreted protein in L. lactis). This gave increased IFN secretion (~3-fold) but lower total production. All the recombinant IFN had appropriate bioactivities in an antiviral assay.
KeywordsHuman interferon alpha Lactococcus lactis LEISSTCDA Secretion
This work was supported by grants from National Program for High Technology Research and Development of China (2006AA10Z319) and the Key Project of the Chinese Academy of Sciences (KSCXZ-YW-G-016). We greatly appreciate Ms. Ling Fu and Dr. Xiaoling Liu for their kindly help for analysis of the biological activity of IFN.
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