N-terminally truncated GADD34 proteins are convenient translation enhancers in a human cell-derived in vitro protein synthesis system
Human cell-derived in vitro protein synthesis systems are useful for the production of recombinant proteins. Productivity can be increased by supplementation with GADD34, a protein that is difficult to express in and purify from E. coli. Deletion of the N-terminal 120 or 240 amino acids of GADD34 improves recovery of this protein from E. coli without compromising its ability to boost protein synthesis in an in vitro protein synthesis system. The use of N-terminally truncated GADD34 proteins in place of full-length GADD34 should improve the utility of human cell-based cell-free protein synthesis systems.
KeywordsCell-free system GADD34 In vitro translation Mammalian cells Protein synthesis
This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (20200056) and a Grant-in-Aid for Scientific Research (C) (19580113) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan to H. I. and by the Targeted Proteins Research Program (TPRP) from MEXT.
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