Biotechnology Letters

, Volume 32, Issue 7, pp 915–920

Novel Escherichia coli hybrids with enhanced butanol tolerance

Original Research Paper

DOI: 10.1007/s10529-010-0247-3

Cite this article as:
Winkler, J., Rehmann, M. & Kao, K.C. Biotechnol Lett (2010) 32: 915. doi:10.1007/s10529-010-0247-3


Hybrids between Escherichia coli and Lactobacillus brevis were generated via protoplast fusion. Growth kinetics of five hybrid strains and E. coli were used to evaluate the butanol tolerance of the novel strains under different conditions. The hybrid strains tolerated up to 2% (v/v) butanol compared to the 1% (v/v) maximum for E. coli. The growth inhibitory effects of butanol were also significantly less in several of the hybrids compared to E. coli. These results demonstrate the potential use of protoplast fusion to generate butanol-tolerant strains.


Butanol tolerance E. coli Intergeneric hybrids L. brevis Protoplast fusion 

Supplementary material

10529_2010_247_MOESM1_ESM.pdf (52 kb)
Supplementary material 1 (PDF 52 kb)
10529_2010_247_MOESM2_ESM.pdf (503 kb)
Supplementary material 2 (PDF 502 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Chemical EngineeringTexas A&M UniversityCollege StationUSA
  2. 2.Department of Chemical and Biomolecular EngineeringUniversity of PennsylvaniaPhiladelphiaUSA

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