Mesenchymal stem cells (MSCs) are candidates for cell therapy of kidney diseases. However, the application of MSC derived from human umbilical cord (UC-MSC) in treating acute renal failure (ARF) has not been reported. UC-MSCs, 106, were transplantated via the left carotid artery into ARF rats which was established by clamping bilateral pedicles for 60 min and reperfusing. Serum creatinine and urea nitrogen decreased 4.8 times and 3.6 times as well as caspase-3 and IL-1β decreased 5.8 times and 9 times compared to control groups, respectively. The percent of proliferative cell nuclear antigen (PCNA)-positive cells (53% ± 7.5%) was higher than that in the control groups (17% ± 4.5%). In addition, the transplanted UC-MSCs could reside in local injury sites, leading to the relief of hyperemia and inflammation, but no obvious transdifferentiation into renal-like cells. The results lay the foundation for further study on the potential application of UC-MSC in human disease.
This is a preview of subscription content, log in to check access.
This work was supported by the National Natural Science Foundation of China, grant no. 30840053, Jiangsu Province’s Outstanding Medical Academic Leader Program, grant no. LJ200614, the Natural Science Foundation of the Jiangsu Province, grant nos. BK2007092, the Sci-Tech innovation team and talents of Jiangsu University (grant no. 2008-018-02), and Foundation of the Jiangsu Province for transfer of scientific and technological achievements(grant no. BA2009124).
Chao KC, Chao KF, Fu YS et al (2008) Islet-like clusters derived from mesenchymal stem cells in Wharton’s Jelly of the human umbilical cord for transplantation to control type 1 diabetes. PLoS One 3:e1451CrossRefPubMedGoogle Scholar
da Silva LB, Palma PV, Cury PM et al (2007) Evaluation of stem cell administration in a model of kidney ischemia-reperfusion injury. Int Immunopharmacol 7:1609–1616CrossRefPubMedGoogle Scholar
Herrera MB, Bussolati B, Bruno S et al (2007) Exogenous mesenchymal stem cells localize to the kidney by means of CD44 following acute tubular injury. Kidney Int 72:430–441CrossRefPubMedGoogle Scholar
Imberti B, Morigi M, Tomasoni S et al (2007) Insulin-like growth factor-1 sustains stem cell mediated renal repair. J Am Soc Nephrol 18:2921–2928CrossRefPubMedGoogle Scholar
Kermani AJ, Fathi F, Mowla SJ (2008) Characterization and genetic manipulation of human umbilical cord vein mesenchymal stem cells: potential application in cell-based gene therapy. Rejuvenation Res 11:379–386CrossRefPubMedGoogle Scholar
Kestendjieva S, Kyurkchiev D, Tsvetkova G et al (2008) Characterization of mesenchymal stem cells isolated from the human umbilical cord. Cell Biol Int 32:724–732CrossRefPubMedGoogle Scholar
Morigi M, Introna M, Imberti B et al (2008) Human bone marrow mesenchymal stem cells accelerate recovery of acute renal injury and prolong survival in mice. Stem Cells 26:2075–2082CrossRefPubMedGoogle Scholar
Neuss S, Becher E, Woltje M et al (2004) Functional expression of HGF and HGF receptor/c-met in adult human mesenchymal stem cells suggests a role in cell mobilization, tissue repair, and wound healing. Stem Cells 22:405–414CrossRefPubMedGoogle Scholar
Qian H, Yang H, Xu W et al (2008) Bone marrow mesenchymal stem cells ameliorate rat acute renal failure by differentiation into renal tubular epithelial-like cells. Int J Mol Med 22:325–332PubMedGoogle Scholar
Qiao C, Xu W, Zhu W et al (2008) Human mesenchymal stem cells isolated from the umbilical cord. Cell Biol Int 32:8–15CrossRefPubMedGoogle Scholar
Semedo P, Palasio CG, Oliveira CD et al (2009) Early modulation of inflammation by mesenchymal stem cell after acute kidney injury. Int Immunopharmacol 9:677–682CrossRefPubMedGoogle Scholar
Togel F, Hu Z, Weiss K et al (2005) Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms. Am J Physiol Renal Physiol 289:F31–F42CrossRefPubMedGoogle Scholar
Togel F, Weiss K, Yang Y et al (2007) Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury. Am J Physiol Renal Physiol 292:F1626–F1635CrossRefPubMedGoogle Scholar
Wang Y, Hu F, Wang ZJ et al (2008) Administration of bone marrow-derived stem cells suppresses cellular necrosis and apoptosis induced by reperfusion of ischaemic kidneys in rats. Chin Med J (Engl) 121:268–271Google Scholar
Wang L, Tran I, Seshareddy K et al (2009) A comparison of human bone marrow-derived mesenchymal stem cells and human umbilical cord-derived mesenchymal stromal cells for cartilage tissue engineering. Tissue Eng Part A 15:2259–2266CrossRefPubMedGoogle Scholar
Wong CY, Cheong SK, Mok PL et al (2008) Differentiation of human mesenchymal stem cells into mesangial cells in post-glomerular injury murine model. Pathology 40:52–57CrossRefPubMedGoogle Scholar
Xu W, Zhang X, Qian H et al (2004) Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med (Maywood) 229:623–631Google Scholar
Yan Y, Xu W, Qian H et al (2009) Mesenchymal stem cells from human umbilical cords ameliorate mouse hepatic injury in vivo. Liver Int 29:356–365CrossRefPubMedGoogle Scholar
Yang CC, Shih YH, Ko MH et al (2008) Transplantation of human umbilical mesenchymal stem cells from Wharton’s jelly after complete transection of the rat spinal cord. PLoS One 3:e3336CrossRefPubMedGoogle Scholar
1.School of Medical Science and Laboratory Medicine, Center for Clinical Laboratory Medicine of Affiliated HospitalJiangsu University, Zhenjiang Key Institute of Clinical Laboratory MedicineZhenjiangPeople’s Republic of China