Encapsulation of recombinant E. coli expressing cyclopentanone monooxygenase in polyelectrolyte complex capsules for Baeyer–Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one
- 267 Downloads
Recombinant Escherichia coli cells, over-expressing cyclopentanone monooxygenase activity, were immobilized in polyelectrolyte complex capsules, made of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl2 and NaCl. More than 90% of the cell viability was preserved during the encapsulation process. Moreover, the initial enzyme activity was fully maintained within encapsulated cells while it halved in free cells. Both encapsulated and free cells reached the end point of the Baeyer–Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one to 4,9-dioxabicyclo[4.2.1]non-7-en-3-one at the same time (48 h). Similarly, the enantiomeric excess above 94% was identical for encapsulated and free cells.
KeywordsBaeyer–Villiger biooxidation Cyclopentanone monooxygenase Encapsulation Immobilization Poly(methylene-co-guanidine)
This work was supported by the Slovak Research and Development Agency under the contract APVV-51-033205, the Slovak Grant Agency for Science VEGA 1/4452/07, COST Action 865 and COST Action CM0701.
- Bučko M, Vikartovská A, Lacík I, Kolláriková G, Gemeiner P, Pätoprstý V, Brygin M (2005) Immobilization of a whole-cell epoxide-hydrolyzing biocatalyst in sodium alginate–cellulose sulfate–poly(methylene-co-guanidine) capsules using a controlled encapsulation process. Enzym Microb Technol 36:118–126CrossRefGoogle Scholar
- Bučko M, Gemeiner P, Vikartovská A, Mislovičová D, Lacík I, Tkáč J (2009) Coencapsulation of oxygen carriers and glucose oxidase in polyelectrolyte complex capsules for the enhancement of d-gluconic acid and δ-gluconolactone production. Artif Cells Blood Substit Biotechnol (accepted)Google Scholar