Abstract
α-Synuclein is a native, unfolded protein that causes several neurodegenerative diseases such as dementia with Lewy bodies and Parkinson’s disease. We have now identified the first DNA aptamers against α-synuclein using native PAGE applied to the SELEX method. We call this aptamer “M5-15”; it is the α-synuclein-bound aptamer and was isolated after four cycles of screening. M5-15 is composed of three stem-loop structures that may play an important role in the binding to α-synuclein. Moreover, M5-15 specifically binds to the α-synuclein monomer and oligomer. We expect that this aptamer will become a useful tool in α-synuclein analysis and diagnosis.
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Tsukakoshi, K., Harada, R., Sode, K. et al. Screening of DNA aptamer which binds to α-synuclein. Biotechnol Lett 32, 643–648 (2010). https://doi.org/10.1007/s10529-010-0200-5
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DOI: https://doi.org/10.1007/s10529-010-0200-5