Biotechnology Letters

, Volume 31, Issue 7, pp 1043–1049 | Cite as

Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions

  • Yanna LiangEmail author
  • Nicolas Sarkany
  • Yi Cui
Original Research Paper


Biomass and lipid productivities of Chlorella vulgaris under different growth conditions were investigated. While autotrophic growth did provide higher cellular lipid content (38%), the lipid productivity was much lower compared with those from heterotrophic growth with acetate, glucose, or glycerol. Optimal cell growth (2 g l−1) and lipid productivity (54 mg l−1 day−1) were attained using glucose at 1% (w/v) whereas higher concentrations were inhibitory. Growth of C. vulgaris on glycerol had a similar dose effects as those from glucose. Overall, C. vulgaris is mixotrophic.


Algal fuels Autotrophic Biodiesel Chlorella vulgaris Glycerol Heterotrophic Lipid productivity 



This research is supported by Southern Illinois University Carbondale (SIUC)’s new faculty start-up funds. Financial support from SIUC Material Technology Center is also greatly acknowledged.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Civil and Environmental EngineeringSouthern Illinois UniversityCarbondaleUSA

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