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Biotechnology Letters

, Volume 31, Issue 6, pp 797–802 | Cite as

Construction and characterization of a high activity mutant of human keratinocyte growth factor-2

  • Jinfeng Wang
  • Xin Cai
  • Minji Zou
  • Tao Xu
  • Shen Liu
  • Yuanyuan Wang
  • Jiaxi Wang
  • Donggang Xu
Original Research Paper

Abstract

Keratinocyte growth factor-2 (KGF-2) plays an important role in vertebrate limb development, lung branching morphogenesis, regeneration and reconstruction of the epidermis. Previous studies have used the wild type factor. Here, we have constructed a double-site mutant of human KGF-2, named STEA. STEA possesses higher receptor binding affinity and promotes better proliferation activity on rat tracheal epithelium (RTE) cells than recombinant human KGF-2. These results suggest that the simultaneous mutation of Ser115 to Thr and Glu117 to Ala improves the biological activity of KGF-2.

Keywords

Cell proliferation Keratinocyte growth factor receptor Keratinocyte growth factor-2 Mutation 

Notes

Acknowledgments

The authors would like to thank Professor Peitang Huang and Dr. Junjie Yue for their helpful suggestions for the design of the KGF-2 mutant.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Jinfeng Wang
    • 1
  • Xin Cai
    • 1
  • Minji Zou
    • 1
  • Tao Xu
    • 1
  • Shen Liu
    • 1
  • Yuanyuan Wang
    • 1
  • Jiaxi Wang
    • 1
  • Donggang Xu
    • 1
  1. 1.Laboratory of Molecular GeneticsBeijing Institute of Basic Medical SciencesBeijingChina

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