Biotechnology Letters

, Volume 31, Issue 5, pp 765–770

Entrapment of cytochrome P450 BM-3 in polypyrrole for electrochemically-driven biocatalysis

  • Dirk Holtmann
  • Klaus-Michael Mangold
  • Jens Schrader
Original Research Paper

DOI: 10.1007/s10529-009-9925-4

Cite this article as:
Holtmann, D., Mangold, KM. & Schrader, J. Biotechnol Lett (2009) 31: 765. doi:10.1007/s10529-009-9925-4

Abstract

In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization of pyrrole proved to be a useful means of immobilising an active cytochrome P450 BM-3 mutein on platinum and glassy carbon electrodes without denaturation. Repeatedly sweeping the electric potential between −600 and +600 mV versus Ag/AgCl led to enzymatically-catalysed product formation while in the absence of the enzyme no product formed under otherwise identical conditions.

Keywords

Cofactor substitution Conductive polymer Cytochrome P450 Electrochemically driven biocatalysis Immobilisation P450 monooxygenase 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Dirk Holtmann
    • 1
  • Klaus-Michael Mangold
    • 2
  • Jens Schrader
    • 1
  1. 1.Biochemical Engineering GroupKarl-Winnacker-InstitutFrankfurt am MainGermany
  2. 2.Electrochemistry GroupKarl-Winnacker-InstitutFrankfurt am MainGermany

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