Entrapment of cytochrome P450 BM-3 in polypyrrole for electrochemically-driven biocatalysis
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- Holtmann, D., Mangold, KM. & Schrader, J. Biotechnol Lett (2009) 31: 765. doi:10.1007/s10529-009-9925-4
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In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization of pyrrole proved to be a useful means of immobilising an active cytochrome P450 BM-3 mutein on platinum and glassy carbon electrodes without denaturation. Repeatedly sweeping the electric potential between −600 and +600 mV versus Ag/AgCl led to enzymatically-catalysed product formation while in the absence of the enzyme no product formed under otherwise identical conditions.