Online monitoring of oxygen in spinner flasks
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We show the application of a novel optical on-line sensor fixed in spinner flasks for the online monitoring of dissolved O2 concentrations during mammalian cell growth. Using this sensor that requires only minute changes to the flask to be made, we could determine the volumetric O2 transfer coefficient as well as O2 consumption rates. Under normal growth conditions the cells did not undergo O2 limitation. Also, the transfer of O2 from the atmosphere to the spinner flasks is influenced by the use of screw caps. The on-line measurement was further applied to determine the O2 uptake rates which can then be used to monitor the metabolic state of the cells and also for online process monitoring.
KeywordsMammalian cell culture Online O2 monitoring Optical oxygen sensor Oxygen transfer Spinner flask
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