Biotechnology Letters

, Volume 32, Issue 3, pp 379–385 | Cite as

Selection of RNA aptamers specific to active prostate-specific antigen

  • Sujin Jeong
  • Seung Ryul Han
  • Young Ju Lee
  • Seong-Wook Lee
Original Research Paper

Abstract

A counter-SELEX procedure with recombinant purified active prostate specific antigen (PSA) was used to identify specific RNA aptamers against the active PSA. We developed two different kinds of counter-SELEX methods; one includes pre-clearance step with inactive proPSA protein, and the other with tagged GST protein. After 9 iterative selection cycles, several identical RNA aptamers can be identified from both counter-SELEX methods. Real-time PCR analysis and gel retardation experiment showed that the aptamers have a specific binding activity against the active PSA, but not for GST or proPSA. These aptamers could be of potential use as specific diagnostic, imaging and/or therapeutic agents against prostate cancer.

Keywords

Prostate cancer Prostate-specific antigen RNA aptamer SELEX 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Sujin Jeong
    • 1
  • Seung Ryul Han
    • 1
  • Young Ju Lee
    • 1
  • Seong-Wook Lee
    • 1
  1. 1.Department of Molecular BiologyInstitute of Nanosensor and Biotechnology, Dankook UniversitySuji-gu, YonginKorea

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