Selection of RNA aptamers specific to active prostate-specific antigen
A counter-SELEX procedure with recombinant purified active prostate specific antigen (PSA) was used to identify specific RNA aptamers against the active PSA. We developed two different kinds of counter-SELEX methods; one includes pre-clearance step with inactive proPSA protein, and the other with tagged GST protein. After 9 iterative selection cycles, several identical RNA aptamers can be identified from both counter-SELEX methods. Real-time PCR analysis and gel retardation experiment showed that the aptamers have a specific binding activity against the active PSA, but not for GST or proPSA. These aptamers could be of potential use as specific diagnostic, imaging and/or therapeutic agents against prostate cancer.
KeywordsProstate cancer Prostate-specific antigen RNA aptamer SELEX
This subject is supported by Korea Ministry of Environment as “The Eco-technopia 21 project” (091-081-073) and Bio Strategic Technology Development of Korea Ministry of Knowledge Economy (10031930).
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