Biotechnology Letters

, Volume 32, Issue 2, pp 243–248 | Cite as

Screening for conditions of enhanced production of a recombinant β-glucanase secreted into the medium by Escherichia coli

  • Meike Spexard
  • Usama BeshayEmail author
  • Joe Max Risse
  • Gerhard Miksch
  • Erwin Flaschel
Original Research Paper


The extracellular production of a hybrid bacterial β-glucanase using Escherichia coli was studied by using combinations of promoters of varying strength for both a β-glucanase as the target protein and the Kil protein as the releasing factor. Four strains with different combinations of promoter strengths were cultivated in shake-flasks on four different media to assess the cross-influence of promoter and medium in a general manner. Promoters were taken from natural as well as synthetic sequences known to exhibit either weak or strong promoter strength. By far the highest extracellular glucanase activity (>200 U ml−1) was achieved when a strain harbouring the kil gene under control of a strong synthetic stationary-phase promoter and the glucanase gene under control of a strong synthetic constitutive promoter was cultivated on a complex medium mainly composed of casein peptone, yeast extract, and glycerol.


β-Glucanase Extracellular enzyme Fermentation E. coli Complex and synthetic media 



U Beshay is grateful to the Alexander von Humboldt-Foundation for the financial support in the form of a fellowship.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Meike Spexard
    • 1
  • Usama Beshay
    • 2
    Email author
  • Joe Max Risse
    • 1
  • Gerhard Miksch
    • 1
  • Erwin Flaschel
    • 1
  1. 1.Fermentation Engineering, Faculty of TechnologyBielefeld UniversityBielefeldGermany
  2. 2.Bioprocess Development DepartmentGenetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research and Technology ApplicationsNew Borg El-Arab City, AlexandriaEgypt

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