Metabolic changes of salicylic acid-elicited Catharanthus roseus cell suspension cultures monitored by NMR-based metabolomics
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The effect of salicylic acid (SA) on the metabolic profile of Catharanthus roseus suspension cells throughout a time course (0, 6, 12, 24, 48 and 72 h after treatment) was investigated using NMR spectroscopy and multivariate data analysis. When compared to control cell lines, SA-treated cells showed a high level of sugars (glucose and sucrose) up to 48 h after treatment, followed by a dynamic change in amino acids, phenylpropanoids, and tryptamine. Additionally, one compound—2,5-dihydroxybenzoic-5-O-glucoside—was detected solely in SA-treated cells.
KeywordsCatharanthus roseus Cell suspension cultures Elicitation Nuclear magnetic resonance spectroscopy-based metabolomics Salicylic acid
Catharanthus roseus is a terpenoid indole-alkaloid (TIA) producing plant. More than 100 TIAs have been identified in this species, including the anti-tumor agents vincristine and vinblastine (dimeric) and the anti-hypertensive ajmalicine. Other simple phenolic metabolites (C6C1) probably involved in the interaction of this plant species with its environment have also been detected (reviewed by Mustafa and Verpoorte 2007). Among these, salicylic acid (SA)—a compound derived from chorismate, the end-product of the shikimate pathway—functions as a signal compound in plants to induce a defense system known as systemic acquired resistance (SAR), a response to a pathogenic attack (Ryals et al. 1996). However, chorismate is the substrate of five different enzymes involved in the production of various secondary metabolites (reviewed by Mustafa and Verpoorte 2005) so that the possibility of distinguishing SA-dependent and independent pathways would contribute to gain more insight in SAR related secondary metabolite production. The level of SA increases in plants attacked by pathogens, e.g., tobacco mosaic virus (Verberne et al. 2000) or Pythium aphanidermatum (Mustafa et al. 2009). Elicitation with Pythium extract also produced an increase of 2,3-dihydroxybenzoic acid (2,3-DHBA; Moreno et al. 1994; Budi Muljono et al. 2002) and tryptamine (Moreno et al. 1996) in C. roseus suspension cells. However, Pythium extract is a mixture of compounds, which may activate different parts of the SAR pathways employing diverse signaling compounds. In order to identify exclusively SA affected metabolites, NMR spectroscopy in combination with multivariate data analysis, a powerful tool for plant metabolite studies, was used. There are numerous successful examples of its application, such as the discrimination between healthy and phytoplasma infected C. roseus leaves (Choi et al. 2004), the metabolic profiling of Passiflora garckei (Fraccaroli et al. 2008) and the differentiation between Tabernaemontanadivaricata cell suspension cultures before and after cryopreservation (Suhartono et al. 2005).
In this study, we analyzed the effect of SA on the metabolite profile of C. roseus cell suspension culture using NMR combined with multivariate data analysis such as principal component analysis (PCA) and partial least square–discriminant analysis (PLS-DA).
Materials and methods
Plant cell cultures
Catharanthus roseus line A12A2 was grown in Murashige and Skoog (1962; M&S) liquid medium without growth hormone and supplemented with 2% (w/v) glucose as a carbon source. The cells were grown in 250 ml Erlenmeyer flasks containing 100 ml medium, cultivated at 24–25°C under continuous light (500–1500 lux) on a shaker at 100 rpm, and subcultured every week by adding the same amount of fresh medium into the cell cultures for maintenance. For the experiment, the suspension cells were subcultured into 100 ml Erlenmeyer flasks (each containing 50 ml medium) in the same cultivation conditions for 5 days prior to elicitation.
Elicitation and harvesting cells
Elicitation with salicylic acid (SA) was achieved by adding 50 μl of a previously 0.2 μm-membrane filtered solution of 0.5 M sodium salicylate to a 100 ml flask containing 50 ml cell suspension culture. As a control, 50 μl sterilized-water was added to 50 ml cell suspension culture. The elicited cells as well as the control cells were harvested at time 0, 6, 12, 24, 48 and 72 h after the addition of the sodium salicylate or the sterile water. Experiments were performed by triplicate both for the elicited cells and the control cells. In the harvesting step, the cells from each flask were rinsed twice with 100 ml of deionized water, vacuum filtered using a P2 glass-filter, transferred to a 10 ml plastic tube, weighed and stored in −80°C. These frozen-cells were freeze-dried in 48 h.
Freeze-dried cells (50 mg) from each flask were placed in a 2 ml micro-tube and extracted with 750 μl CH3OH-d4 and 750 μl KH2PO4-d2 buffer in D2O (pH 6.0). The mixture was vortexed (2,500 rpm, 1 min) using an electronic vortex mixer followed by sonication (20 min) in an ultrasonic bath and centrifugation (10,000×g, 15 min). The supernatant was transferred into a 5 mm NMR tube for NMR measurements.
1H-NMR and J-resolved spectra were recorded at 25°C on a 400 MHz Bruker AV-400 spectrometer operating at proton NMR frequency of 400.13 MHz. CH3OH-d4 was used as an internal lock. Each spectrum consisted of 128 scans requiring 10 min acquisition time with the following parameters: 0.25 Hz/point, pulse width (PW) = 45º (6.6 μs), and relaxation delay (Dl) = 2.0 s. A presaturation sequence was used to suppress the residual water signal with low power selective irradiation at the water frequency during the recycle delay. FIDs were Fourier transformed with LB = 0.3 Hz and the spectra were zero-filled to 32 K points. The window functions were optimized for the analysis. The resulting spectra were manually phased and baseline corrected, and calibrated to TMSP at 0.0 ppm, always using XWIN NMR (version 3.5, Bruker). Two dimensional J-resolved 1H-NMR spectra were acquired using 8 scans per 32 increments that were collected into 16 K data points, using spectral widths of 5.208 KHz in F2 (chemical shift axis) and 50 Hz in F1 (spin–spin coupling constant axis). A 1 s relaxation delay was employed, giving a total acquisition time of 14.52 min. Datasets were zero-filled to 512 points in F1 and both dimensions were multiplied by sine-bell functions prior to double complex FT. J-resolved spectra tilted by 45° were symmetrized about F1 and then calibrated, always using XWIN NMR (version 3.5 Bruker). Data were exported as the 1 D projection (F2 axis) of the 2D J-resolved spectra.
The 1H-NMR and F2-projected J-resolved spectra were automatically reduced to ASCII files using AMIX (v. 3.7, Bruker Biospin). Spectral intensities were scaled to TMSP and reduced to integrated regions of equal width (0.04 ppm) corresponding to the region of δ −0.4 to δ 10. Principal component analyses (PCA) partial least square-discriminant analysis (PLS-DA) were performed with the SIMCA-P software (v. 12.0, Umetrics, Umeå, Sweden).
Results and discussion
Characteristic chemical shifts (δ in ppm) and coupling constants (J) of some compounds detected in the 1H-NMR and J-resolved 1H-NMR spectra (CH3OH-d4-KH2PO4 in D2O, pH 6.0, 1:1) of control- and SA-treated cells of Catharanthus roseus suspension culture
Chemical shift (ppm) and coupling constant (Hz)
δ 0.97 (d, J = 6.5 Hz); δ 0.98 (d, J = 6.7 Hz)
δ 0.95 (t, J = 7.5 Hz); δ 1.02 (d, J = 6.8 Hz)
δ 1.00 (H-γ, d, J = 7.0 Hz); δ 1.05 (H-γ′, d, J = 7.0 Hz)
δ 1.33 (H-γ, d, J = 6.6 Hz)
δ 1.48 (H-β, d, J = 7.2 Hz)
δ 2.46 (H-γ, m), δ 2.13 (H-β, m)
δ 2.82 (H-, dd, J1 = 8.1 Hz, J2 = 17.0 Hz);
δ 2.95 (H-, dd, J1 = 3.8 Hz, J2 = 16.8 Hz);
δ 3.94 (H-, dd, J1 = 3.8 Hz, J2 = 8.4 Hz)
δ 5.18 (α-form, H-1, d, J = 3.8 Hz), δ 4.58 (β-form, H-1, d, J = 7.8 Hz),
δ 5.40 (H-1, d, J = 3.8 Hz); δ 4.17 (H-1’, d, J = 8.6 Hz)
δ 6.54 (s)
δ 7.36 (m)
Salicylic acid (2-hydroxybenzoic acid)
δ 6.88 (H-3, d, J = 8 Hz); δ 6.90 (H-5, t, J = 7.5 Hz);
δ 7.37 (H-4, t, J = 7.5 Hz); δ 7.81 (H-6, d, J = 8 Hz)
Gentisic acid glucoside (2,5-dihydroxybenzoic acid glucoside)
δ 6.83 (H-3, d, J = 9 Hz); δ 7.17 (H-4, dd, J1 = 9 Hz, J2 = 3 Hz); δ 7.56 (H-6, d, J = 3 Hz); δ 4.88 (H-1’, d, J = 7.5 Hz)
Among the elicited groups, initial time (0 h) treated cells had the highest positive effect on PC1 which decreased with time, indicating the occurrence of an immediate change, such as pH, oxidative-burst or glucosidase activity after elicitation as compared to the control groups (0, 12 and 24 h), after which it normalized again (24 h after elicitation) to produce a second change in the longer term (48–72 h after elicitation), probably dependent on gene-induction. Either SA or JA (jasmonic acid) or other signal compounds are capable of inducing such a change. The change in the metabolic profile observed in this study followed a pattern comparable to that observed in gene-expression or enzyme activity in C. roseus suspension cultures treated by JA or Pythium extract reported by other groups (van der Fits and Memelink 2001; Pasquali et al. 1992; Moreno et al. 1996). Van der Fits and Memelink (2001) showed that elicitation of C. roseus cells with methyl jasmonate (MeJA) rapidly induced a regulator gene ORCA3 already within 0–2 h peaking at 0.5 h, whilst the mRNA of the structural genes, e.g., TDC and STR responsible for tryptophan decarboxylase (TDC) and strictosidine synthase (STR) were strongly induced later, 2–8 h after elicitation with a maximum at 8 h. Pasquali et al. (1992) also reported that C. roseusTDC and STR were induced 8 h after elicitation with extracts from yeast or P. aphanidermatum and Moreno et al. (1996) showed that an increase in anthranilate synthase (AS) activity from 2 to around 8 nkatl−1 occurred more than 10 h after elicitation, whereas TDC activity increased from around 20 to 90 nkatl−1 between 20 and 30 h after elicitation with Pythium extract. Strictosidine synthase and strictosidine-β-glucosidase activities were slightly increased compared to control cells. Budi Muljono et al. (2002) observed a metabolic change through time in C. roseus cells elicited by Pythium extract, noting that the largest increase of the level of endogenous SA in the cells occurred 8 h after elicitation, whilst the highest increase of the level of 2,3-DHBA in the cells was found 20 h after elicitation and 24 h after elicitation in the medium.
All these previous results prove that different phases of cell metabolism occur upon elicitation. The phases can be distinguished as biochemical changes (phase 1), the alteration of gene expression (phase 2) and the switching on of the defense response/metabolite production (phase 3). The present study also confirms the existence of such a pattern of different phases in the C. roseus cells metabolism upon elicitation.
Fumaric acid was present at low levels at 0–24 h in both the control and treated cells (Fig. 3) but, while a slight increase was observed from 48 to 72 h in control cells, a significant increase occurred in elicited cells in the same period, probably as a storage product due to a down-regulation of the Krebs cycle. Fumaric acid is an intermediate in the Krebs cycle that can be converted into malic acid, oxaloacetate and subsequently to phosphoenolpyruvate (PEP), a precursor of shikimate and chorismate. Some enzymes in the Krebs cycle are down-regulated if the level of energy (ATP) in the cell is already high, thus no excess oxaloacetate would be available to enter the Krebs cycle (Berg et al. 2002) resulting consequently in a high concentration of fumaric acid .
Phenylalanine was detected in both control and SA-treated cells. In the SA-treated cells the minimum level of phenylalanine occurred at 6 h after treatment, increasing again (16-fold) to reach the highest level at 72 h.
The almost total conversion of fed SA into gentisic acid glucoside by C. roseus suspension cells has been reported (Shimoda et al. 2002). However, in our experiments the levels of gentisic acid glucoside in the cells represented only 10% of the fed SA. As the media were not analyzed, we cannot exclude the possibility that the amounts of benzoic acid derivatives present in the media would complete the mass balance of the SA bioconversion. These results do not provide sufficient evidence to conclude that gentisic acid glucoside is formed from SA though, considering the results of Shimoda et al. (2002), it could be expected that this is the case, being produced by oxidation of SA followed by glucosylation. The signals of gentisic acid glucoside appeared weakly at 24 h and strongly increased in intensity at 48 and 72 h.
In the case of other metabolites, such as amino acids, maximum levels of valine, alanine, aspartic acid and fumaric acid were detected at 6 h after elicitation, whilst leucine and isoleucine showed minimum levels at that same time. In this initial 6 h period, the pyruvate pool might increase through glycolysis, the Krebs cycle and the catabolism of leucine and isoleucine, generating an increase in the valine, alanine, aspartic acid and fumaric acid levels. At 12 h after elicitation, the levels of the amino acids and organic acids (leucine, isoleucine, valine, threonine, aspartic acid and fumaric acid except glutamic acid and alanine) were back again to the initial levels, but subsequently increased again to reach another maximum at 48–72 h. This could imply that from 12 to 48 h, the pyruvate pool probably increased again in order to provide the precursors required for the synthesis of secondary metabolites.
Glutamate level in control cells remained constant throughout all the experiment, but decreased slowly in elicited cells (Fig. 3e). Glutamate is necessary for ammonia (NH3) assimilation to prevent the build-up in the cell of toxic ammonia, which is produced by the reduction of nitrate (NO3−), nitrogen fixation or from the catabolism of other amino acids. Ammonia assimilation by glutamate leads to glutamine, which can be transaminated to obtain glutamate again when the amide amino group of glutamine is transferred to other compounds. The enzymes responsible for this, (glutamine synthetase, EC 126.96.36.199 and glutamate synthase EC 188.8.131.52 or EC 184.108.40.206) are involved in the glutamate synthase cycle. Glutamate can also be converted to proline and arginine.
In summary, application of NMR spectroscopy in combination with PCA and PLS-DA on the extracts of C. roseus suspension cells clearly revealed an altered-metabolite profiles of SA-treated cells as compared to control cells during a period of 0–72 h. SA signals already appeared weakly at 0 h and were detected clearly at 6–24 h. Sugars (glucose and sucrose) were found in increased-levels at 0–24 h in the SA-treated cells compared to the control cells (highest levels at 0 h), but depletion of sugars occurred at 48–72 h in both control- and SA-treated cells. The levels of some aliphatic amino acids and organic acids in the SA-treated cells showed two maxima at 6 h and 48 h. The fed SA disappeared after 48 h, but at the same time gentisic acid glucose (2,5-DHBAG) signals appeared. Overall the metabolic response exhibited two phases, an immediate change of the metabolome followed by a partly return to normal conditions and a subsequent second major change in the metabolome. The first phase could probably represent an immediate biochemical response, followed by a second response at gene level.
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