Biotechnology Letters

, 31:1947 | Cite as

Continuous cultivation approach for fermentative succinic acid production from crude glycerol by Basfia succiniciproducens DD1

Original Research Paper

Abstract

A continuous cultivation process for the fermentative production of succinic acid from glycerol with the recently isolated bacterium Basfia succiniciproducens DD1 was developed. Crude glycerol (5.1 g l−1) was used as C-source and NH4OH as N-source and pH-control agent. The problem of wall growth was solved by transfers of the cultivation broth into an empty identical fermentor. The resulting continuous cultivation process was maintained for more than 80 days. Glycerol-limited steady states were established for dilution rates between 0.004 and 0.018 h−1. Higher dilution rates resulted in glycerol accumulation. Succinic acid concentrations, productivities, yields and specific productivities increased with increasing dilution rates: at 0.018 h−1 the highest values were 5.21 g l−1, 0.094 g l−1 h−1, 1.02 g g−1 and 0.375 g g−1 h−1, respectively.

Keywords

Basfia succiniciproducens Continuous cultivation Crude glycerol Glycerol Pasteurellaceae Succinic acid 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Regulatory Ecotoxicology Chemicals, BASF SELudwigshafen am RheinGermany
  2. 2.Fermentation Products Research, BASF SELudwigshafen am RheinGermany
  3. 3.Osthus GmbHMannheimGermany

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