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Biotechnology Letters

, 31:1639 | Cite as

Advances in enzyme immobilisation

  • Dean Brady
  • Justin Jordaan
Review

Abstract

Improvements in current strategies for carrier-based immobilisation have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit improved protein binding capacity. Novel methods of enzyme self-immobilisation have been developed (CLEC, CLEA, Spherezyme), as well as carrier materials (Dendrispheres), encapsulation (PEI Microspheres), and entrapment. Apart from retention, recovery and stabilisation, other advantages to enzyme immobilisation have emerged, such as enhanced enzyme activity, modification of substrate selectivity and enantioselectivity, and multi-enzyme reactions. These advances promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications.

Keywords

Biocatalyst Biocatalysis Enzyme Immobilisation 

Notes

Acknowledgements

We would like to thank BioPAD and ZA Biotech for financial support in development of SphereZymes, and Novozymes SA for provision of enzymes in ongoing research projects.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Enzyme TechnologiesCSIR BiosciencesModderfonteinSouth Africa
  2. 2.Department of Biotechnology and Food TechnologyTshwane University of TechnologyPretoriaSouth Africa
  3. 3.Molecular BiomaterialsCSIR BiosciencesBrummeriaSouth Africa

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