Biotechnology Letters

, 31:1775 | Cite as

Stress responses to heterologous membrane protein expression in Escherichia coli

Original Research Paper

Abstract

The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms.

Keywords

G protein-coupled receptors Heterologous protein expression Sigma factor Stress response 

Notes

Acknowledgments

This research was supported by Princeton University startup funds and by the Lidow Senior Thesis fund. The authors thank Dr. Eva-Maria Strauch (University of Washington) for comments on the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Departments of Chemical EngineeringPrinceton UniversityPrincetonUSA
  2. 2.Departments of Molecular BiologyPrinceton UniversityPrincetonUSA

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