Biotechnology Letters

, Volume 31, Issue 4, pp 571–576 | Cite as

Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells

  • Mongkol Thirabunyanon
  • Pongphun Boonprasom
  • Piyanuch Niamsup
Original Research Paper

Abstract

Fifty-four strains of lactic acid bacteria obtained from fermented dairy milks were investigated for possible use as probiotics and for colon cancer biological products. Five of these strains inhibited growth of eight food-borne pathogens including Helicobacter pylori, Escherichia coli, and Salmonella typhimurium. Three of these strains survived at pH 2.5 and in 0.3% bile salts. Additionally they produced no haemolysis, were resistant to kanamycin and adhered to Caco-2 cells. 16S rRNA gene sequences of probiotic strains indicated that RM11 and RM28 were Enterococcus faecium and Lactobacillus fermentum, respectively. Both the cultured medium and live whole cells from probiotic strains were tested for antiproliferation of colon cancer cells through MTT and Trypan Blue exclusion assays. The probiotic strains of E. faecium RM11 and L. fermentum RM28 also triggered antiproliferation of colon cancer cells at the rates of 21–29%, and 22–29%, respectively. This suggested that both strains could be used as potential probiotics in functional food or for colon cancer biological products.

Keywords

Caco-2 cells Colon cancer Fermented milk Lactic acid bacteria Probiotic 

Notes

Acknowledgements

Financial support for this research was provided by the National Research Council of Thailand.

Supplementary material

10529_2008_9902_MOESM1_ESM.doc (31 kb)
Supplementary material 1 (DOC 31 kb)

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mongkol Thirabunyanon
    • 1
  • Pongphun Boonprasom
    • 1
  • Piyanuch Niamsup
    • 1
  1. 1.Biotechnology Section, Faculty of ScienceMaejo UniversityChiang MaiThailand

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