Biotechnology Letters

, Volume 31, Issue 4, pp 571–576 | Cite as

Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells

  • Mongkol ThirabunyanonEmail author
  • Pongphun Boonprasom
  • Piyanuch Niamsup
Original Research Paper


Fifty-four strains of lactic acid bacteria obtained from fermented dairy milks were investigated for possible use as probiotics and for colon cancer biological products. Five of these strains inhibited growth of eight food-borne pathogens including Helicobacter pylori, Escherichia coli, and Salmonella typhimurium. Three of these strains survived at pH 2.5 and in 0.3% bile salts. Additionally they produced no haemolysis, were resistant to kanamycin and adhered to Caco-2 cells. 16S rRNA gene sequences of probiotic strains indicated that RM11 and RM28 were Enterococcus faecium and Lactobacillus fermentum, respectively. Both the cultured medium and live whole cells from probiotic strains were tested for antiproliferation of colon cancer cells through MTT and Trypan Blue exclusion assays. The probiotic strains of E. faecium RM11 and L. fermentum RM28 also triggered antiproliferation of colon cancer cells at the rates of 21–29%, and 22–29%, respectively. This suggested that both strains could be used as potential probiotics in functional food or for colon cancer biological products.


Caco-2 cells Colon cancer Fermented milk Lactic acid bacteria Probiotic 



Financial support for this research was provided by the National Research Council of Thailand.

Supplementary material

10529_2008_9902_MOESM1_ESM.doc (31 kb)
Supplementary material 1 (DOC 31 kb)


  1. Arici M, Bilgin B, Sagdic O, Ozdemir C (2004) Some characteristics of Lactobacillus isolates from infant faeces. Food Microbiol 21:19–24CrossRefGoogle Scholar
  2. Baccigalupi L, Donato AD, Parlato M, Luongo D, Carbone V, Rossi M, Ricca E, Felice MD (2005) Small surface-associated factors mediate adhesion of a food-isolate strain of Lactobacillus fermentum to Caco-2 cells. Res Microbiol 156:830–836PubMedCrossRefGoogle Scholar
  3. Commane D, Hughes R, Shortt C, Rowland I (2005) The potential mechanisms involved in the anti-carcinogenic action of probiotics. Mutat Res 591:276–289PubMedGoogle Scholar
  4. Ewaschuk JB, Walker JW, Diaz H, Madsen KL (2006) Bioproduction of conjugated linoleic acid by probiotic bacteria occurs in vitro and in vivo in mice. J Nutr 136:1483–1487PubMedGoogle Scholar
  5. Gonet-Surowka AK, Strus M, Heczko PB (2007) Influence of Lactobacilli probiotic strains on apoptosis of colon cancer cells lines. Int J Antimicrob Agents 29:S343–S344CrossRefGoogle Scholar
  6. Leroy F, Moreno MRF, Vuyst LD (2003) Enterococcus faecium RZS C5, an interesting bacteriocin producer to be used as a co-culture in food fermentation. Int J Food Microbiol 88:235–240PubMedCrossRefGoogle Scholar
  7. Maragkoudakis PA, Zoumpopoulor G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E (2006) Probiotic potential of Lactobacillus strains isolated from dairy products. Int Dairy J 16:189–199CrossRefGoogle Scholar
  8. Moreno D, LeBlanc DA, Matar C, Perdigón G (2007) The application of probiotics in cancer. Br J Nutr 98 (Suppl 1):S105–S110Google Scholar
  9. Pennacchia C, Ercolini D, Blaiotta G, Pepe O, Mauriello G, Villani F (2004) Selection of Lactobacillus strains from fermented sausages for their potential use as probiotics. Meat Sci 67:309–317CrossRefGoogle Scholar
  10. Rafter J (2003) Probiotics and colon cancer. Best Pract Res Clin Gastroenterol 17:849–859PubMedCrossRefGoogle Scholar
  11. Saikali J, Picard C, Freitas M, Holt P (2004) Fermented milks, probiotic cultures, and colon cancer. Nutr Cancer 49:14–24PubMedCrossRefGoogle Scholar
  12. Salminen S, Wright A, Morelli L, Marteuau P, Brassart D, Vos WM, Fonden R, Saxelin M, Collins K, Mogensen G, Birkeland SE, Mattila-Sandholm T (1998) Demonstration of safety of probiotics—a review. Int J Food Microbiol 44:93–106PubMedCrossRefGoogle Scholar
  13. Shmuely H, Passaro D, Figer A, Niv Y, Pitlik S, Samra Z, Koren R, Yahav J (2001) Relationship between Helicobacter pylori CagA status and colorectal cancer. Am J Gastroenterol 96:3406–3410PubMedCrossRefGoogle Scholar
  14. Travaglione S, Fabbri A, Fiorentini C (2008) The Rho-activating CNF1 toxin from pathogenic E. coli: A risk factor for human cancer development? Infect Agent Cancer 3:4PubMedCrossRefGoogle Scholar
  15. Zoumpopoulou G, Foligne B, Christodoulou K, Grangette C, Pot B, Tsakalidou E (2008) Lactobacillus fermentum ACA-DC 179 displays probiotic potential in vitro and protects against trinitrobenzene sulfonic acid (TNBS)-induced colitis and Salmonella infection in murine models. Int J Food Microbiol 121:18–26PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mongkol Thirabunyanon
    • 1
    Email author
  • Pongphun Boonprasom
    • 1
  • Piyanuch Niamsup
    • 1
  1. 1.Biotechnology Section, Faculty of ScienceMaejo UniversityChiang MaiThailand

Personalised recommendations