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Biotechnology Letters

, Volume 31, Issue 4, pp 607–614 | Cite as

Production of imidazole alkaloids in cell cultures of jaborandi as affected by the medium pH

  • N. L. Andreazza
  • I. N. Abreu
  • A. C. H. F. Sawaya
  • M. N. Eberlin
  • P. Mazzafera
Original Research Paper

Abstract

The effect of pH (from 4.8 to 9.8) on the production of pilosine and pilocarpine and on their partition between cell and medium was studied in two lineages (P and PP) of Pilocarpus microphyllus cell suspension cultures. Highest mass accumulation was observed at high pHs and both lineages produced pilocarpine while only lineage PP produced pilosine. Both alkaloids were released in the medium but higher accumulation occurred in the cells. The highest production of pilocarpine was at pH 8.8–9.8 in both cell lineages. Other imidazole alkaloids were also identified in both lineages. At all pHs tested, the pH in the media cultures tended to stabilize around 6 after 10–15 days of cultivation. NO3 and NH4 + variation in the media might partially explain the pH stabilization.

Keywords

Medium pH Membrane transporters Pilocarpine Pilocarpus microphyllus Pilosine 

Notes

Acknowledgements

N.L.A., I.N.A. and A.C.H.F.S. thank the Conselho Nacional de Desenvolvimento Científico Tecnológico do Brasil (CNPq-Brasil) and the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for student fellowships. M.N.E. and P.M. thank CNPq-Brasil for research fellowships. This work was partially supported by FAPESP.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • N. L. Andreazza
    • 1
  • I. N. Abreu
    • 1
    • 2
  • A. C. H. F. Sawaya
    • 3
  • M. N. Eberlin
    • 3
  • P. Mazzafera
    • 1
  1. 1.Departamento de Fisiologia Vegetal, Instituto de BiologiaUniversidade Estadual de CampinasCampinasBrazil
  2. 2.Plant Physiology DepartmentUmeå UniversityUmeåSweden
  3. 3.Instituto de QuímicaUniversidade Estadual de CampinasCampinasBrazil

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